Document Detail


Differential kinetics of [123I]beta-CIT binding to dopamine and serotonin transporters.
MedLine Citation:
PMID:  8612664     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Iodine-123-labelled 3beta-(4-iodophenyl)tropane-2beta-carboxylic acid ([123I]beta-CIT) labels both the dopamine transporter (DAT) and the serotonin transporter (5-HTT) and this ligand is able to clarify pathological changes in both dopaminergic and serotonergic systems. However, the differential kinetics of beta-CIT binding to DAT and 5-HTT has not been clarified fully. In this study we examined time-activity curves of [123I]beta-CIT in individual regions in the rat brain. Using cerebellum as the reference region, k3 and k4 values were estimated by a two-compartment kinetic analysis. In the striatum, the kinetics was slowest among all brain areas. In this area specific binding reached its peak 4 h after the injection. In the hypothalamus, specific binding reached its peak 1 h after the injection and its amount did not change until 4 h after the injection. In the occipital cortex, the binding and washout of the ligand were fastest among all brain regions. Estimated k3 values were 0.040+/-0.003 in the striatum, 0.019+/-0.002 in the hypothalamus and 0.082+/-0.011 in the occipital cortex (min-1, mean +/-SD). Estimated k4 values were 0.0034+/-0.0005 in the striatum, 0.0071+/-0.0009 in the hypothalamus and 0.083+/-0.013 in the occipital cortex (min-1, mean +/-SD). Therefore binding kinetics of [123i]beta-cit in the region rich in dat is apparently different from that in the region rich in 5-HTT. These results will provide fundamental data to image both DAT and 5-HTT in one series of examinations with [123I]beta-CIT.
Authors:
M Fujita; K Takatoku; Y Matoba; M Nishiura; K Kobayashi; O Inoue; T Nishimura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of nuclear medicine     Volume:  23     ISSN:  0340-6997     ISO Abbreviation:  Eur J Nucl Med     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-06-06     Completed Date:  1996-06-06     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7606882     Medline TA:  Eur J Nucl Med     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  431-6     Citation Subset:  IM    
Affiliation:
Division of Tracer Kinetics, Biomedical Research Center, Osaka University Medical School, Osaka 565, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Chemistry
Carrier Proteins / metabolism*
Cocaine / analogs & derivatives*,  diagnostic use,  pharmacokinetics
Dopamine Plasma Membrane Transport Proteins
Iodine Radioisotopes / diagnostic use*
Kinetics
Male
Membrane Glycoproteins / metabolism*
Membrane Transport Proteins*
Nerve Tissue Proteins*
Rats
Serotonin / metabolism
Serotonin Plasma Membrane Transport Proteins
Tissue Distribution
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Dopamine Plasma Membrane Transport Proteins; 0/Iodine Radioisotopes; 0/Membrane Glycoproteins; 0/Membrane Transport Proteins; 0/Nerve Tissue Proteins; 0/Serotonin Plasma Membrane Transport Proteins; 0/Slc6a3 protein, rat; 0/Slc6a4 protein, rat; 133647-95-7/RTI 55; 50-36-2/Cocaine; 50-67-9/Serotonin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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