| Differential ion accumulation and ion fluxes in the mesophyll and epidermis of barley. | |
MedLine Citation:
|
PMID: 10712547 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In barley (Hordeum vulgare L.) leaves, differential ion accumulation commonly results in inorganic phosphate (Pi) being confined to the mesophyll and Ca(2+) to the epidermis, with preferential epidermal accumulation of Cl(-), Na(+), and some other ions. The pattern was confirmed in this study for major inorganic anions and cations by analysis of barley leaf protoplasts. The work focused on the extent to which differences in plasma membrane ion transport processes underlie these observations. Ion transport across the plasma membrane of barley epidermal and mesophyll protoplasts was investigated electrophysiologically (by microelectrode impalement and patch clamping) and radiometrically. Data from both approaches suggested that similar types of ion-selective channels and membrane transporters, which catalyze the transport of Ca(2+), K(+), Na(+), and Pi, exist in the plasma membrane of the two cell types. In general, the simple presence or absence of ion transporters could not explain cell-type-specific differences in ion accumulation. However, patch-clamp data suggested that differential regulation of instantaneously activating ion channels in the plasma membrane could explain the preferential accumulation of Na(+) in the epidermis. |
Authors:
|
A J Karley; R A Leigh; D Sanders |
Related Documents
:
|
15877 - Light-dependent cation gradients and electrical potential in halobacterium halobium cel... 1333147 - Altered stoichiometry of the na,k-atpase. 1447087 - Continuous measurement of na concentration in csf during gastric water infusion in dehy... 2921327 - Effects of acetazolamide on na+-hco-3 cotransport in basolateral membrane vesicles isol... 14667 - Active proton uptake by chromaffin granules: observation by amine distribution and phos... 12794677 - Temperature and meal size effects on the postprandial metabolism and energetics in a bo... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Plant physiology Volume: 122 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 2000 Mar |
Date Detail:
|
Created Date: 2000-05-25 Completed Date: 2000-05-25 Revised Date: 2013-04-16 |
Medline Journal Info:
|
Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 835-44 Citation Subset: IM |
Affiliation:
|
Department of Biology, University of York, P.O. Box 373, York YO10 5YW, United Kingdom. ajk9@york.ac.uk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Calcium
/
metabolism Cell Membrane / metabolism Hordeum / metabolism* Ion Channels / metabolism Ion Transport Membrane Potentials Patch-Clamp Techniques Phosphates / metabolism Plant Leaves / metabolism Potassium / metabolism Protoplasts / metabolism Sodium / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Ion Channels; 0/Phosphates; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A weakly voltage-dependent, nonselective cation channel mediates toxic sodium influx in wheat.
Next Document: Import of lyso-phosphatidylcholine into chloroplasts likely at the origin of eukaryotic plastidial l...