Document Detail


Differential interaction of retroviral vector with target cell: quantitative effect of cellular receptor, soluble proteoglycan, and cell type on gene delivery efficiency.
MedLine Citation:
PMID:  18620488     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Retroviral vectors are powerful tools for gene therapy and stem cell engineering. To improve efficiency of retroviral gene delivery, quantitative understanding of interactions of a retroviral vector and a cell is crucial. Effects of nonspecific adsorption of retrovirus on a cell via proteoglycans and receptor-mediated binding of retrovirus to a cell on overall transduction efficiency were quantified by combining a mathematical model and experimental data. Results represented by transduction rate constant, a lumped parameter of overall transduction efficiency, delineated that chondroitin sulfate C (CSC) plays dual roles as either enhancer or inhibitor of retroviral transduction, depending on its concentrations in the retroviral supernatant. At the concentration of 20 microg/mL, CSC enhanced the transduction efficiency up to threefold but inhibited more than sevenfold at the concentration of 100 microg/mL. Transduction rate constants for amphotropic retroviral infection of NIH 3T3 cells under phosphate-depleted culture condition showed a proportional relationship between cellular receptor density on a cell and transduction efficiency. It was finally shown that amphotropic retrovirus transduced human fibroblast HT1080 cells more efficiently than NIH 3T3 cells. On the contrary, the transduction efficiency of NIH 3T3 cells by vesicular stomatitis virus G protein pseudotyped retroviruses was eightfold higher than that of HT1080 cells. This study implies usefulness of using quantitative analysis of retroviral transduction in understanding and optimizing retroviral gene delivery systems for therapeutic approaches to tissue engineering.
Authors:
Young Jik Kwon; Ching-An Peng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  14     ISSN:  1937-3341     ISO Abbreviation:  -     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-08     Completed Date:  2009-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1497-506     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, University of Southern California, Los Angeles, California 92697, USA. kwonyj@uci.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding, Competitive / drug effects
Cell Line
Cell Proliferation / drug effects
Cell Shape / drug effects
Chondroitin Sulfates / pharmacology
Genetic Vectors / genetics,  metabolism
Humans
Mice
NIH 3T3 Cells
Phosphates / pharmacology
Proteoglycans / pharmacology
Receptors, Virus / metabolism
Retroviridae / genetics,  metabolism*
Transduction, Genetic / methods*
Chemical
Reg. No./Substance:
0/Phosphates; 0/Proteoglycans; 0/Receptors, Virus; 9007-28-7/Chondroitin Sulfates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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