| Differential interaction of Aggregatibacter (Actinobacillus) actinomycetemcomitans LsrB and RbsB proteins with autoinducer 2. | |
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MedLine Citation:
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PMID: 17526716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our previous studies showed that the Aggregatibacter actinomycetemcomitans RbsB protein interacts with cognate and heterologous autoinducer 2 (AI-2) signals and suggested that the rbsDABCK operon encodes a transporter that may internalize AI-2 (D. James et al., Infect. Immun. 74:4021-4029, 2006.). However, A. actinomycetemcomitans also possesses genes related to the lsr operon of Salmonella enterica serovar Typhimurium which function to import AI-2. Here, we show that A. actinomycetemcomitans LsrB protein competitively inhibits the interaction of the Vibrio harveyi AI-2 receptor (LuxP) with AI-2 from either A. actinomycetemcomitans or V. harveyi. Interestingly, LsrB was a more potent inhibitor of LuxP interaction with AI-2 from V. harveyi whereas RbsB competed more effectively with LuxP for A. actinomycetemcomitans AI-2. Inactivation of lsrB in wild-type A. actinomycetemcomitans or in an isogenic RbsB-deficient strain reduced the rate by which intact bacteria depleted A. actinomycetemcomitans AI-2 from solution. Consistent with the results from the LuxP competition experiments, the LsrB-deficient strain depleted AI-2 to a lesser extent than the RbsB-deficient organism. Inactivation of both lsrB and rbsB virtually eliminated the ability of the organism to remove AI-2 from the extracellular environment. These results suggest that A. actinomycetemcomitans possesses two proteins that differentially interact with AI-2 and may function to inactivate or facilitate internalization of AI-2. |
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Authors:
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Hanjuan Shao; Deanna James; Richard J Lamont; Donald R Demuth |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-05-25 |
Journal Detail:
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Title: Journal of bacteriology Volume: 189 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-07-19 Completed Date: 2007-09-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 5559-65 Citation Subset: IM |
Affiliation:
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Department of Periodontics, Endodontics and Dental Hygiene, University of Louisville School of Dentistry, 501 South Preston Street, Room 209, Louisville, KY 40292, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actinobacillus actinomycetemcomitans
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metabolism* Bacterial Proteins / antagonists & inhibitors, metabolism* Gene Deletion Homoserine / analogs & derivatives*, metabolism Lactones / metabolism* Periplasmic Binding Proteins / metabolism* Protein Binding Receptors, Cell Surface / metabolism* Vibrio |
| Grant Support | |
ID/Acronym/Agency:
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R01 DE14605/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Lactones; 0/LuxP protein, Vibrio; 0/N-octanoylhomoserine lactone; 0/Periplasmic Binding Proteins; 0/Receptors, Cell Surface; 498-19-1/Homoserine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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