| Differential integrin expression by cardiac fibroblasts from hypertensive and exercise-trained rat hearts. | |
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MedLine Citation:
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PMID: 11934598 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cardiac fibroblast is the principal cell type responsible for extracellular matrix (ECM) synthesis in the heart during growth and pathophysiological conditions. A dynamic interaction exists between the cardiac ECM and fibroblasts that is sensitive to the local mechanical and chemical tissue environment. We propose here that cardiac fibroblasts structurally and functionally adapt to changing local environments by altering their expression of receptor integrins. Changes in the extracellular environment are communicated in part by integrins, which link the ECM to the cell and regulate phenotype and function. In this report, we analyze integrin protein expression, migration and gel contraction by cardiac fibroblasts from rats subjected to 10 weeks of treadmill exercise (XTR), experimental hypertension (HYP) or controls (CONT). Immunoprecipitation shows that beta1 protein increases in XTR and HYP. Also, alpha1 and alpha2 integrins are lower in XTR and HYP, and alpha5 integrin is higher in XTR and lower in HYP. Functional assays show that XTR and HYP migrate slower on collagen, while XTR migrate faster and HYP slower on fibronectin. Cell isolation procedure, population expansion number or a general adaptation to culture conditions does not explain the differences observed. No significant differences in collagen gel contraction are detected. These results indicate that cardiac fibroblasts retain their in vivo patterns in vitro for a limited number of population expansions. This tissue-specific phenotype is exhibited in early passage (< or =6). However, by late passage (>8), cells begin to show adaptation to the in vitro conditions. These results show that cardiac fibroblasts respond to changing environments in pathophysiological conditions by modulating integrin expression, which is associated with changes in cell migration. They also suggest a pragmatic use for primary cardiac fibroblasts as a model to study the cardiac matrix remodeled by physiological (exercise) and pathological (hypertension) stressors. |
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Authors:
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Maria Lonnett Burgess; Louis Terracio; Toshiro Hirozane; Thomas K Borg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology Volume: 11 ISSN: 1054-8807 ISO Abbreviation: Cardiovasc. Pathol. Publication Date: 2002 Mar-Apr |
Date Detail:
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Created Date: 2002-04-05 Completed Date: 2002-07-24 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9212060 Medline TA: Cardiovasc Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 78-87 Citation Subset: IM |
Affiliation:
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Laboratory of Cardiovascular Biology, Department of Health Sciences, Boston University, 675 Commonwealth Ave., Boston, MA 02215, USA. mburgess@bu.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Movement Cells, Cultured Collagen / metabolism Fibroblasts / cytology, metabolism* Gels Heart Ventricles / metabolism* Hypertension / metabolism*, pathology Integrins / metabolism* Motor Activity / physiology* Myocardium / cytology, metabolism Physical Conditioning, Animal Physical Exertion / physiology* Rats |
| Grant Support | |
ID/Acronym/Agency:
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AG 14535/AG/NIA NIH HHS; HL-08713-01/HL/NHLBI NIH HHS; HL-24935/HL/NHLBI NIH HHS; HL-40424/HL/NHLBI NIH HHS; HL-43582/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Gels; 0/Integrins; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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