Document Detail


Differential inhibition of attachment and eclipse activities of HeLa cells for enteroviruses.
MedLine Citation:
PMID:  4306196     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Receptor activities of HeLa cells were evaluated for ability to both attach and eclipse enteroviruses after exposure of cells to acid or heat. A modified procedure of acid (pH 1.5) elution of cell-associated virus, as compared with other procedures, provided a general method for the optimal recovery of receptor-bound enteroviruses. With this procedure, eclipse of virus operationally was considered to be that amount of virus infectivity which was determined initially to be cell-associated and which was not dissociable from the cells. HeLa cells killed by heating at 56 C for 30 min could not attach or eclipse poliovirus T1, but they attached and eclipsed coxsackieviruses B1 and B3, and they attached echovirus 6 but did not eclipse it. HeLa cells treated at pH 2.5 for 10 min at 2 C could not attach or eclipse poliovirus T1, but they attached coxsackieviruses B1 and B3 and echovirus 6, although these viruses were not eclipsed. These results showed that, within the operational definition of virus eclipse, the eclipse activity of HeLa cells for some viruses can be irreversibly inactivated without impairing the activity of the receptors for attaching these viruses. The data provided additional evidence that HeLa cells possess specific receptors for the different enteroviruses.
Authors:
I Zajac; R L Crowell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  3     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1969 Apr 
Date Detail:
Created Date:  1969-07-08     Completed Date:  1969-07-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  422-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Binding Sites*
Chymotrypsin / pharmacology
Detergents / pharmacology
Enterovirus*
Enterovirus B, Human*
Freezing
Glycine / pharmacology
Hela Cells* / drug effects
Hot Temperature
Hydrogen-Ion Concentration
Lithium / pharmacology
Poliovirus*
Urea / pharmacology
Virus Replication
Chemical
Reg. No./Substance:
0/Detergents; 56-40-6/Glycine; 57-13-6/Urea; 7439-93-2/Lithium; EC 3.4.21.1/Chymotrypsin
Comments/Corrections

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