Document Detail


Differential in vivo gene expression of major Leptospira proteins in resistant or susceptible animal models.
MedLine Citation:
PMID:  22729538     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transcripts of Leptospira 16S rRNA, FlaB, LigB, LipL21, LipL32, LipL36, LipL41, and OmpL37 were quantified in the blood of susceptible (hamsters) and resistant (mice) animal models of leptospirosis. We first validated adequate reference genes and then evaluated expression patterns in vivo compared to in vitro cultures. LipL32 expression was downregulated in vivo and differentially regulated in resistant and susceptible animals. FlaB expression was also repressed in mice but not in hamsters. In contrast, LigB and OmpL37 were upregulated in vivo. Thus, we demonstrated that a virulent strain of Leptospira differentially adapts its gene expression in the blood of infected animals.
Authors:
Mariko Matsui; Marie-Estelle Soupé; Jérôme Becam; Cyrille Goarant
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-22
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-10     Completed Date:  2012-12-11     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6372-6     Citation Subset:  IM    
Affiliation:
Institut Pasteur de Nouvelle-Calédonie, Institut Pasteur International Network, Bacterial Research Laboratory, Noumea, New Caledonia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / biosynthesis*
Blood / microbiology
Cricetinae
Disease Models, Animal
Disease Resistance
Gene Expression Profiling
Gene Expression Regulation, Bacterial*
Host-Pathogen Interactions
Leptospira / genetics*
Leptospirosis / microbiology*
Mice
RNA, Ribosomal, 16S / biosynthesis
Virulence
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/RNA, Ribosomal, 16S
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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