| Differential impact of copper deficiency in rats on blood cuproproteins. | |
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MedLine Citation:
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PMID: 19700037 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sensitive blood biochemical markers of dietary copper status are not yet known. Rat models were used to investigate the response of severe copper deficiency in dams and pups by comparing abundance of several cuproproteins in erythrocytes, white blood cells, and platelets. The hypothesis tested was that copper deficiency would result in changes in abundance of cuproproteins in blood cells. Copper-deficient (CuD) Holtzman dams and pups had signs consistent with severe copper deficiency compared with copper-adequate controls including lower liver copper and hemoglobin levels and near total loss of plasma ceruloplasmin diamine oxidase activity. Copper-deficient erythrocytes had lower copper, zinc superoxide dismutase (SOD1) but higher copper metallochaperone for SOD1 (CCS) compared with copper-adequate, resulting in higher CCS/SOD1 levels. This ratio was more sensitive in CuD erythrocytes than CuD white cells and especially in CuD platelets. However, both white blood cells and platelets from CuD dams and pups had nearly nondetectable levels of cytochrome c oxidase subunit IV. Because isolation of relatively pure populations of erythrocytes and platelets is feasible, and reagents for immunoblot methods are available, determination of CCS/SOD1 and cytochrome c oxidase subunit IV protein levels may be useful to assess copper status of humans. |
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Authors:
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Margaret A Broderius; Joseph R Prohaska |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Nutrition research (New York, N.Y.) Volume: 29 ISSN: 1879-0739 ISO Abbreviation: - Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-08-24 Completed Date: 2009-10-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8303331 Medline TA: Nutr Res Country: United States |
Other Details:
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Languages: eng Pagination: 494-502 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, University of Minnesota Medical School, Duluth, MN 55812, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amine Oxidase (Copper-Containing)
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blood* Animals Biological Markers / blood Blood Platelets / metabolism Blood Proteins / metabolism Ceruloplasmin / metabolism* Copper / deficiency*, metabolism Electron Transport Complex IV / blood* Erythrocytes / metabolism* Hemoglobins / metabolism* Iron / metabolism Leukocytes / metabolism Liver / metabolism Molecular Chaperones Rats Rats, Sprague-Dawley Superoxide Dismutase / blood* Zinc / blood |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Blood Proteins; 0/Hemoglobins; 0/Molecular Chaperones; 7439-89-6/Iron; 7440-50-8/Copper; 7440-66-6/Zinc; EC 1.15.1.1/Superoxide Dismutase; EC 1.16.3.1/Ceruloplasmin; EC 1.4.3.6/Amine Oxidase (Copper-Containing); EC 1.9.3.1/Electron Transport Complex IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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