| Differential hydrolysis of erythrocyte and mitochondrial membrane phospholipids by two phospholipase A2 isoenzymes (NK-PLA2-I and NK-PLA2-II) from the venom of the Indian monocled cobra Naja kaouthia. | |
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MedLine Citation:
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PMID: 15081888 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We previously demonstrated that venom from the Indian monocled cobra Naja kaouthia is a rich source of phospholipase A2 enzymes, and we purified and characterized a major PLA2 isoenzyme (NK-PLA2-I) from N. kaouthia venom. In the present study, we report the purification and biochemical characterization of a second PLA2 isoenzyme (NK-PLA2-II) from the same venom. A comparison of the membrane phospholipid hydrolysis patterns by these two PLA2s has revealed that they cause significantly more damage to mitochondrial membranes (NK-PLA2-I > NK-PLA2-II) as compared to erythrocyte membranes due to more efficient binding of the enzymes to mitochondrial membranes. Fatty acid release patterns by these PLA2s from the membrane phospholipid PC-pools indicate that NK-PLA2-I does not discriminate between saturated and unsaturated fatty acids whereas NK-PLA2-II shows a preference for unsaturated fatty acids during the initial phase of attack. The current investigation provides new insight into the molecular arrangement of NK-PLA2-sensitive domains in erythrocyte and mitochondrial membranes and highlights the contribution of polar, but uncharged, amino acids such as serine and cysteine in NK-PLA2 induced membrane damage. |
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Authors:
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Robin Doley; Glenn F King; Ashis K Mukherjee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 425 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2004 May |
Date Detail:
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Created Date: 2004-04-14 Completed Date: 2004-05-19 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: United States |
Other Details:
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Languages: eng Pagination: 1-13 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784 028, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antivenins / pharmacology Cell Membrane / drug effects, metabolism* Cobra / metabolism* Cobra Venoms / metabolism* Erythrocytes / drug effects, metabolism Isoenzymes / isolation & purification, metabolism Mitochondria / drug effects, metabolism* Phospholipases A / drug effects, isolation & purification, metabolism* Phospholipases A2 Phospholipids / metabolism Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antivenins; 0/Cobra Venoms; 0/Isoenzymes; 0/Phospholipids; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
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