Document Detail

Differential homing commitments of antigen-specific T cells after oral or parenteral immunization in humans.
MedLine Citation:
PMID:  10227989     Owner:  NLM     Status:  MEDLINE    
Animal experiments show that lymphocytes activated in the intestine circulate through mesenteric lymph nodes, lymphatics, and blood, returning to the gut. Homing into intestinal lamina propria is mediated by lymphocyte surface homing receptors, mainly the alpha4beta7-integrin. We studied in humans whether intestinal T cells entering the blood upon antigenic activation would exhibit homing commitments to the gut. Volunteers were immunized with keyhole limpet hemocyanin (KLH) first orally and then parenterally or only parenterally, and the expression of alpha4beta7 on T cells specific for KLH or tetanus toxoid was studied. Circulating T cells were depleted of alpha4beta7+ cells by immunomagnetic selection. This depletion removed a significant proportion of the KLH-specific cells (mean decrease in proliferative response of 71%) in the orally immunized volunteers. No difference in the KLH-induced proliferation was found between the total and the alpha4beta7-depleted populations in volunteers parenterally immunized with KLH, regardless of whether a preceding mucosal priming had taken place or not. In both immunization groups, the depletion of alpha4beta7+ cells had no influence on the proliferative response to tetanus toxoid. We conclude that, in contrast to T cells activated by parenteral immunization, gut-derived T cells have preferential homing commitments to the gut. This commitment was no longer observed after a subsequent parenteral Ag administration. Besides showing that the site of Ag encounter determines the expression of homing receptors, the present study is the first to provide evidence for a circulation of newly activated Ag-specific intestinal T cells back to the gut in humans.
A Kantele; J Zivny; M Häkkinen; C O Elson; J Mestecky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  162     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-05-20     Completed Date:  1999-05-20     Revised Date:  2013-11-14    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5173-7     Citation Subset:  AIM; IM; X    
University of Alabama, Birmingham, AL 35294, USA.
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MeSH Terms
Administration, Oral
CD4-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / immunology
Cell Movement / immunology*
Epitopes, T-Lymphocyte / physiology*
Hemocyanin / administration & dosage,  immunology*
Injections, Subcutaneous
Integrins / biosynthesis
Lymphocyte Activation / immunology
T-Lymphocytes / cytology*,  immunology*,  metabolism
Tetanus Toxoid / administration & dosage,  immunology*
Grant Support
Reg. No./Substance:
0/Epitopes, T-Lymphocyte; 0/Integrins; 0/Tetanus Toxoid; 0/integrin alpha4beta7; 9013-72-3/Hemocyanin; FV4Y0JO2CX/keyhole-limpet hemocyanin

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