Document Detail

Differential gene and protein expression in abluminal sprouting and intraluminal splitting forms of angiogenesis.
MedLine Citation:
PMID:  16402918     Owner:  NLM     Status:  MEDLINE    
In adult skeletal muscle, abluminal sprouting or longitudinal splitting of capillaries can be initiated separately by muscle overload and elevated microcirculation shear stress respectively. In the present study, gene and protein expression patterns associated with the different forms of angiogenesis were examined using a targeted gene array (Superarray), validated by quantitative RT (reverse transcription)-PCR and immunoblots. Sprouting angiogenesis induced large changes in expression levels in genes associated with extracellular matrix remodelling, such as MMP-2 (matrix metalloproteinase-2), TIMP (tissue inhibitor of metalloproteinases), SPARC (secreted protein, acidic and rich in cysteine) and thrombospondin. Changes in neuropilin, midkine and restin levels, which may underpin changes in endothelial morphology, were seen during splitting angiogenesis. Up-regulation of VEGF (vascular endothelial growth factor), Flk-1, angiopoietin-2 and PECAM-1 (platelet/endothelial cell adhesion molecule-1) was seen in both forms of angiogenesis, representing a common angiogenic response of endothelial cells. In conclusion, the present study demonstrates that general angiogenic signals from growth factors can be influenced by the local microenvironment resulting in differing forms of capillary growth to produce a co-ordinated expansion of the vascular bed.
James L Williams; Alexander Weichert; Andreas Zakrzewicz; Luis Da Silva-Azevedo; Axel R Pries; Oliver Baum; Stuart Egginton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical science (London, England : 1979)     Volume:  110     ISSN:  0143-5221     ISO Abbreviation:  Clin. Sci.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-04-06     Completed Date:  2006-06-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905731     Medline TA:  Clin Sci (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  587-95     Citation Subset:  IM    
Angiogenesis Research Group, Division of Cardiovascular Sciences, The Medical School, The University of Birmingham, Birmingham B15 2TT, UK.
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MeSH Terms
Angiogenic Proteins / genetics,  metabolism
Capillaries / anatomy & histology,  pathology
Gene Expression Regulation
Mice, Inbred C57BL
Muscle Proteins / genetics,  metabolism
Muscle, Skeletal / blood supply*,  physiopathology
Neovascularization, Pathologic / genetics,  metabolism*,  pathology,  physiopathology
Neovascularization, Physiologic / genetics,  physiology*
Protein Array Analysis / methods
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction / methods
Stress, Mechanical
Reg. No./Substance:
0/Angiogenic Proteins; 0/Muscle Proteins; 0/RNA, Messenger

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