Document Detail


Differential gene expression in normal and transformed human mammary epithelial cells in response to oxidative stress.
MedLine Citation:
PMID:  21397008     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxidative stress plays a key role in breast carcinogenesis. To investigate whether normal and malignant breast epithelial cells differ in their responses to oxidative stress, we examined the global gene expression profiles of three cell types, representing cancer progression from a normal to a malignant stage, under oxidative stress. Normal human mammary epithelial cells (HMECs), an immortalized cell line (HMLER-1), and a tumorigenic cell line (HMLER-5) were exposed to increased levels of reactive oxygen species (ROS) by treatment with glucose oxidase. Functional analysis of the metabolic pathways enriched with differentially expressed genes demonstrated that normal and malignant breast epithelial cells diverge substantially in their response to oxidative stress. Whereas normal cells exhibit the up-regulation of antioxidant mechanisms, cancer cells are unresponsive to the ROS insult. However, the gene expression response of normal HMECs under oxidative stress is comparable to that of the malignant cells under normal conditions, indicating that altered redox status is persistent in breast cancer cells, which makes them resistant to increased generation of ROS. We discuss some of the possible adaptation mechanisms of breast cancer cells under persistent oxidative stress that differentiate them from normal mammary epithelial cells as regards the response to acute oxidative stress.
Authors:
Diego F Cortes; Wei Sha; Valerie Hower; Greg Blekherman; Reinhard Laubenbacher; Steven Akman; Suzy V Torti; Vladimir Shulaev
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-03-29
Journal Detail:
Title:  Free radical biology & medicine     Volume:  50     ISSN:  1873-4596     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-09     Completed Date:  2011-09-22     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1565-74     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / genetics,  metabolism*,  pathology,  physiopathology
Carcinoma / genetics,  metabolism*,  pathology,  physiopathology
Cell Line, Transformed
Cell Transformation, Neoplastic* / genetics
Female
Gene Expression Profiling / methods
Glucose Oxidase / metabolism
Humans
Mammary Glands, Human / metabolism*,  pathology
Microarray Analysis
Oxidative Stress* / genetics
Reactive Oxygen Species / metabolism
Grant Support
ID/Acronym/Agency:
R01 CA120170-01A2/CA/NCI NIH HHS; R01 CA120170-02/CA/NCI NIH HHS; R01 CA120170-03/CA/NCI NIH HHS; R01CA120170/CA/NCI NIH HHS; T32 CA079448/CA/NCI NIH HHS; T32 CA079448/CA/NCI NIH HHS; T32 CA079448-05S1/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species; EC 1.1.3.4/Glucose Oxidase
Comments/Corrections

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