Document Detail


Differential expression of vitamin E and selenium-responsive genes by disease severity in chronic obstructive pulmonary disease.
MedLine Citation:
PMID:  23875740     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antioxidant nutritional status is hypothesized to influence chronic obstructive pulmonary disease (COPD) susceptibility and progression. Although past studies relate antioxidants to gene expression, there are no data in patients with COPD. This study investigated the hypothesis that antioxidant status is compromised in patients with COPD, and antioxidant-responsive genes differentially express in a similar pattern. Lung tissue samples from patients with COPD were assayed for vitamin E and gene expression. Selenium and vitamin E were assayed in corresponding plasma samples. Discovery based genome-wide expression analysis compared moderate, severe, and very severe COPD (GOLD II-IV) patients to mild and at-risk/normal (GOLD 0-I). Hypotheses-driven analyses assessed differential gene expression by disease severity for vitamin E-responsive and selenium-responsive genes. GOLD II-IV COPD patients had 30% lower lung tissue vitamin E levels compared to GOLD 0-I participants (p = 0.0082). No statistically significant genome-wide differences in expression by disease severity were identified. Hypothesis-driven analyses of 109 genes found 16 genes differentially expressed (padjusted < 0.05) by disease severity including 6 selenium-responsive genes (range in fold-change -1.39 to 2.25), 6 vitamin E-responsive genes (fold-change -2.30 to 1.51), and 4 COPD-associated genes. Lung tissue vitamin E in patients with COPD was associated with disease severity and vitamin E-responsive genes were differentially expressed by disease severity. Although nutritional status is hypothesized to contribute to COPD risk, and is of therapeutic interest, evidence to date is mainly observational. The findings reported herein are novel, and support a role of vitamin E in COPD progression.
Authors:
Anne H Agler; Ronald G Crystal; Jason G Mezey; Jennifer Fuller; Chuan Gao; Joyanna G Hansen; Patricia A Cassano
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  COPD     Volume:  10     ISSN:  1541-2563     ISO Abbreviation:  COPD     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-07-23     Completed Date:  2014-05-16     Revised Date:  2014-06-23    
Medline Journal Info:
Nlm Unique ID:  101211769     Medline TA:  COPD     Country:  England    
Other Details:
Languages:  eng     Pagination:  450-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Antioxidants / analysis,  metabolism
Female
Gene Expression*
Gene Expression Profiling*
Humans
Lung / chemistry
Male
Middle Aged
Nutritional Status
Oligonucleotide Array Sequence Analysis
Pulmonary Disease, Chronic Obstructive / blood*,  genetics*,  physiopathology
RNA / analysis
Selenium / blood*
Severity of Illness Index
alpha-Tocopherol / analysis,  blood*
Grant Support
ID/Acronym/Agency:
P50 HL084936/HL/NHLBI NIH HHS; P50 HL084936/HL/NHLBI NIH HHS; R03 HL095414/HL/NHLBI NIH HHS; R03 HL095414/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antioxidants; 63231-63-0/RNA; H4N855PNZ1/alpha-Tocopherol; H6241UJ22B/Selenium
Comments/Corrections

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