Document Detail

Differential expression of vasoactive mediators in microparticle-challenged lungs of chickens that differ in susceptibility to pulmonary arterial hypertension.
MedLine Citation:
PMID:  19907003     Owner:  NLM     Status:  MEDLINE    
Pulmonary hypertension syndrome (PHS; ascites) in fast growing meat-type chickens (broilers) is characterized by the onset of idiopathic pulmonary arterial hypertension (IPAH) leading to right-sided congestive heart failure and terminal ascites. Intravenous microparticle (MP) injection is a tool used by poultry geneticists to screen for the broilers that are resistant (RES) or susceptible (SUS) to IPAH in a breeding population. MPs occlude pulmonary arterioles and initiate focal inflammation, causing local tissues and responding leukocytes to release vasoactive mediators such as serotonin (5-HT), endothelin-1 (ET-1), and nitric oxide (NO). RT-PCR was used to examine the differences between RES and SUS broilers in terms of gene expression of ET-1, ET receptor types A and B (ET(A) and ET(B)), the serotonin transporter (SERT), serotonin receptors (5-HT(1A), 5-HT(2A), 5-HT(1B), 5-HT(2B)), endothelial NO synthase (eNOS), and inducible NOS (iNOS) in the lungs of these broilers before (0 h) and after (2, 6, 12, 24, and 48 h) MP injection. In SUS broilers MP injection elicited higher (P < 0.05) pulmonary expression of 5-HT(1A), 5-HT(2B), and ET-1, which promote vasoconstriction and proliferation of pulmonary arterial smooth muscle cells (PASMC). In RES broilers the MP injection elicited higher expression of eNOS, iNOS, and ET(B), which promote vasodilation and inhibit PASMC proliferation. These observations support the hypothesis that the resistance of broiler chickens to IPAH may be due to the higher expression of vasoactive mediators that favor enhanced vasodilation and attenuated vasoconstriction during MP injection challenges to the pulmonary vasculature.
Krishna R Hamal; Robert F Wideman; Nicholas B Anthony; Gisela F Erf
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Publication Detail:
Type:  Journal Article     Date:  2009-11-11
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  298     ISSN:  1522-1490     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-24     Completed Date:  2010-01-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R235-42     Citation Subset:  IM    
Dept. of Poultry Science, Univ. of Arkansas, POSC-420, 1260 W. Maple, Fayetteville, AR 72701, USA.
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MeSH Terms
Disease Susceptibility / diagnosis,  metabolism,  veterinary*
Endothelin-1 / metabolism*
Hypertension, Pulmonary / genetics,  metabolism,  veterinary*
Injections, Intravenous
Lung / blood supply,  metabolism*,  physiopathology
Mass Screening / methods
Nitric Oxide / metabolism*
Nitric Oxide Synthase Type II / metabolism
Nitric Oxide Synthase Type III / metabolism
Poultry Diseases / diagnosis,  genetics,  metabolism*
Pulmonary Artery / physiopathology
Receptors, Endothelin / metabolism
Receptors, Serotonin / metabolism
Serotonin / metabolism*
Vasoconstriction / physiology
Vasodilation / physiology
Reg. No./Substance:
0/Endothelin-1; 0/Receptors, Endothelin; 0/Receptors, Serotonin; 10102-43-9/Nitric Oxide; 50-67-9/Serotonin; EC Oxide Synthase Type II; EC Oxide Synthase Type III

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