Document Detail


Differential expression of two pro-opiomelanocortin mRNAs during temperature stress in common carp (Cyprinus carpio L.).
MedLine Citation:
PMID:  9795345     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pro-opiomelanocortin (POMC) is the precursor of a number of biologically active peptides, including adrenocorticotropic hormone, alpha-melanocyte-stimulating hormone and beta-endorphin, which are released by the pituitary glands of fish as well as mammals. To quantify the levels of expression of the two POMC mRNAs relative to one another during the response of the common carp to temperature-induced stress, we used reverse transcriptase PCR combined with capillary electrophoresis and laser-induced fluorescence detection. The ratio of POMC-I mRNA to POMC-II mRNA determined in wild-type and four isogenic carp strains was found to be strain-dependent and influenced by temperature. In strain E20xR8, the ratio had altered in favour of POMC-I from 1:3.2 (POMC-I:POMC-II) in fish adapted to 24 degreesC to 1:1.2 in fish adapted to a decrease of 9 degreesC in ambient temperature. A rapid drop in temperature from 24 to 15 degreesC decreased the POMC mRNA ratio at the expense of POMC-I from 1:1.9 in the control fish (strain E4xR3R8) to 1:4.2 3 h after the temperature drop of 9 degreesC. We conclude that both POMC genes are expressed in the common carp and that their expression ratio is strain-dependent and changes in response to ambient temperature.
Authors:
R J Arends; R van der Gaag; G J Martens; S E Wendelaar Bonga; G Flik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  159     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-01-29     Completed Date:  1999-01-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  85-91     Citation Subset:  IM    
Affiliation:
Department of Animal Physiology, University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological*
Animals
Carps / metabolism*
Cold Temperature*
Electrophoresis, Capillary
Female
Gene Expression
Hydrocortisone / analysis
Male
Microscopy, Fluorescence
Pituitary Gland / metabolism*
Pro-Opiomelanocortin / genetics*
RNA, Messenger / analysis*
Radioimmunoassay
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/RNA, Messenger; 50-23-7/Hydrocortisone; 66796-54-1/Pro-Opiomelanocortin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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