Document Detail

Differential expression of two flavonoid 3'-hydroxylase cDNAs involved in biosynthesis of anthocyanin pigments and 3-deoxyanthocyanidin phytoalexins in sorghum.
MedLine Citation:
PMID:  16943219     Owner:  NLM     Status:  MEDLINE    
Three unique sorghum flavonoid 3'-hydroxylase (F3'H) cDNAs (SbF3'H1, SbF3'H2 and SbF3'H3) were discovered through bioinformatics analysis. Their encoded proteins showed >60% identity to the Arabidopsis TT7 (F3'H) protein. Overexpression of SbF3'H1 or SbF3'H2 restored the ability of tt7 mutants to produce 3'-hydroxylated flavonoids, establishing their roles as functional F3'H enzymes. In sorghum mesocotyls, SbF3'H1 expression was involved in light-specific anthocyanin accumulation while SbF3'H2 expression was involved in pathogen-specific 3-deoxyanthocyanidin synthesis. No SbF3'H3 expression was detected in all tissues examined. The sorghum mesocotyls represent a good system for investigation of differential regulation of F3'H genes/alleles responding to different external stimuli.
Chun-Hat Shih; Ivan K Chu; Wing Kin Yip; Clive Lo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-30
Journal Detail:
Title:  Plant & cell physiology     Volume:  47     ISSN:  0032-0781     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-30     Completed Date:  2007-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1412-9     Citation Subset:  IM    
Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ787855;  DQ787856;  DQ787857
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MeSH Terms
Amino Acid Sequence
Anthocyanins / biosynthesis,  chemistry
Cytochrome P-450 Enzyme System / biosynthesis,  genetics*
DNA, Complementary / analysis
Gene Expression Regulation, Enzymologic*
Gene Expression Regulation, Plant*
Genes, Plant
Mixed Function Oxygenases / biosynthesis,  genetics*
Molecular Sequence Data
Molecular Structure
Sorghum / genetics*
Terpenes / chemistry,  metabolism
Reg. No./Substance:
0/Anthocyanins; 0/DNA, Complementary; 0/Terpenes; 37297-20-4/phytoalexins; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases; EC 3'-hydroxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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