| Differential expression of syntaxin 1A and 1B by noradrenergic and adrenergic chromaffin cells. | |
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MedLine Citation:
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PMID: 14570390 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The expression and localization of syntaxin isoforms 1A and 1B in adrenergic and noradrenergic chromaffin cells were examined by both immunoblot analysis and confocal immunofluorescence microscopy. Syntaxin 1A was found in higher levels in noradrenergic cells, whereas syntaxin 1B was similarly expressed in most noradrenergic and adrenergic cells. However, some heterogeneity was observed within each catecholaminergic phenotype. Although the majority of adrenergic cells appeared to express low levels of syntaxin 1A, about 7% was strongly stained for syntaxin 1A. A subpopulation of noradrenergic cells, about 17%, expressed greater levels of syntaxin 1B. Syntaxin 1B labeling showed a punctate appearance in the cytoplasm, whereas syntaxin 1A appeared predominantly localized to the plasma membrane. These data show differences in the exocytotic machinery of the two subtypes of chromaffin cells that may underlie some of the distinct characteristics of adrenaline and noradrenaline secretion. |
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Authors:
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Graça Baltazar; Arsélio P Carvalho; Emília P Duarte |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neurochemical research Volume: 28 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2003-10-22 Completed Date: 2003-11-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: United States |
Other Details:
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Languages: eng Pagination: 1453-7 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Centre for Neuroscience and Cell Biology of Coimbra, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Glands
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cytology Animals Antigens, Surface / metabolism* Cattle Cells, Cultured Chromaffin Cells / metabolism* Epinephrine / metabolism* Microscopy, Confocal Microscopy, Fluorescence Nerve Tissue Proteins / metabolism* Norepinephrine / metabolism* Phenotype Protein Isoforms / metabolism Syntaxin 1 |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Surface; 0/Nerve Tissue Proteins; 0/Protein Isoforms; 0/Syntaxin 1; 51-41-2/Norepinephrine; 51-43-4/Epinephrine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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