Document Detail


Differential expression of syntaxin 1A and 1B by noradrenergic and adrenergic chromaffin cells.
MedLine Citation:
PMID:  14570390     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expression and localization of syntaxin isoforms 1A and 1B in adrenergic and noradrenergic chromaffin cells were examined by both immunoblot analysis and confocal immunofluorescence microscopy. Syntaxin 1A was found in higher levels in noradrenergic cells, whereas syntaxin 1B was similarly expressed in most noradrenergic and adrenergic cells. However, some heterogeneity was observed within each catecholaminergic phenotype. Although the majority of adrenergic cells appeared to express low levels of syntaxin 1A, about 7% was strongly stained for syntaxin 1A. A subpopulation of noradrenergic cells, about 17%, expressed greater levels of syntaxin 1B. Syntaxin 1B labeling showed a punctate appearance in the cytoplasm, whereas syntaxin 1A appeared predominantly localized to the plasma membrane. These data show differences in the exocytotic machinery of the two subtypes of chromaffin cells that may underlie some of the distinct characteristics of adrenaline and noradrenaline secretion.
Authors:
Graça Baltazar; Arsélio P Carvalho; Emília P Duarte
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemical research     Volume:  28     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-22     Completed Date:  2003-11-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1453-7     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Centre for Neuroscience and Cell Biology of Coimbra, Portugal.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adrenal Glands / cytology
Animals
Antigens, Surface / metabolism*
Cattle
Cells, Cultured
Chromaffin Cells / metabolism*
Epinephrine / metabolism*
Microscopy, Confocal
Microscopy, Fluorescence
Nerve Tissue Proteins / metabolism*
Norepinephrine / metabolism*
Phenotype
Protein Isoforms / metabolism
Syntaxin 1
Chemical
Reg. No./Substance:
0/Antigens, Surface; 0/Nerve Tissue Proteins; 0/Protein Isoforms; 0/Syntaxin 1; 51-41-2/Norepinephrine; 51-43-4/Epinephrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Regulation of vesicle traffic and neurotransmitter release in isolated nerve terminals.
Next Document:  Regulation of AMPA receptor activity, synaptic targeting and recycling: role in synaptic plasticity.