Document Detail

Differential expression of renal nitric oxide synthase isoforms during pregnancy in rats.
MedLine Citation:
PMID:  9931143     Owner:  NLM     Status:  MEDLINE    
Alterations in nitric oxide (NO) production have been suggested to play a role in mediating changes in renal function during normal pregnancy and in pregnancy-induced hypertension. Although NO production is enhanced during normal pregnancy, the mechanisms for the increase are unknown. The purpose of this study was to determine whether the elevation in NO production during pregnancy is associated with increases in renal expression of endothelial (eNOS), inducible (iNOS), and neuronal (nNOS) nitric oxide synthases. To achieve this goal we examined systemic and renal hemodynamics, urinary excretion of nitrate/nitrite, and renal protein expression of the three NOS isoforms in prepregnant rats, pregnant rats at days 6, 13, and 19 of gestation and at day 4 postpartum. Mean arterial pressure decreased by 14% in late pregnancy whereas the glomerular filtration rate and renal plasma flow increased by 21% and 24%, respectively, in mid pregnancy. Excretion of nitrate/nitrite increased throughout pregnancy with a 3.4-fold increase present at day 19 (12.2+/-0.7 to 41.1+/-1.3 micromol/24 h). Renal eNOS protein expression decreased by 39% during pregnancy with the lowest level resulting at day 19 and returning to virgin levels by day 4 post partum. In contrast, renal iNOS and nNOS protein expression increased 31% and 25%, respectively, with highest expression occurring for both at day 13 of pregnancy. These data suggest that the increased NO production and renal hemodynamics associated with pregnancy in rats may be caused by the upregulation of iNOS and nNOS in the kidney.
B T Alexander; M T Miller; S Kassab; J Novak; J F Reckelhoff; W C Kruckeberg; J P Granger
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hypertension     Volume:  33     ISSN:  0194-911X     ISO Abbreviation:  Hypertension     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-02-26     Completed Date:  1999-02-26     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  435-9     Citation Subset:  IM    
Department of Physiology and Biophysics, Center for Excellence in Cardiovascular-Renal Research Center, University of Mississippi Medical Center, Jackson 39216-4505, USA.
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MeSH Terms
Arginine / blood
Blood Pressure
Gene Expression Regulation, Enzymologic*
Hemodynamics / physiology*
Kidney / enzymology*
Nitrates / urine
Nitric Oxide Synthase / genetics*
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type III
Nitrites / urine
Pregnancy, Animal / physiology*
Rats, Sprague-Dawley
Renal Circulation / physiology*
Grant Support
Reg. No./Substance:
0/Nitrates; 0/Nitrites; 74-79-3/Arginine; EC Oxide Synthase; EC Oxide Synthase Type I; EC Oxide Synthase Type II; EC Oxide Synthase Type III; EC protein, rat; EC protein, rat; EC protein, rat

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