Document Detail


Differential expression of receptors for Shiga and Cholera toxin is regulated by the cell cycle.
MedLine Citation:
PMID:  11865037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cholera and Shiga toxin bind to the cell surface via glycolipid receptors GM1 and Gb3, respectively. Surprisingly, the majority of Vero cells from a non-synchronized population bind either Cholera or Shiga toxin but not both toxins. The hypothesis that the differential expression of toxin receptors is regulated by the cell cycle was tested. We find that Cholera toxin binds preferentially in G0/G1, with little binding through S-phase to telophase, whereas Shiga toxin binds maximally through G2 to telophase but does not bind during G0/G1 and S-phase. The changes result from the corresponding changes in Gb3 and GM1 synthesis, not from variations of receptor transport to the cell surface. The changes do not reflect competition of Gb3 and GM1 synthesis for lactosylceramide. Cells as diverse as Vero cells, PC12 cells and astrocytes show the same cell-cycle-dependent regulation of glycosphingolipid receptors, suggesting that this novel phenomenon is based on a conserved regulatory mechanism.
Authors:
Irina Majoul; Tobias Schmidt; Maria Pomasanova; Evgenia Boutkevich; Yuri Kozlov; Hans-Dieter Söling
Related Documents :
17127697 - Lack of specific binding of shiga-like toxin (verocytotoxin) and non-specific interacti...
6253287 - Influence of cholera toxin on in vitro refractoriness to thyrotropin of thyroids from r...
19652967 - Helicoverpa armigera cadherin fragment enhances cry1ac insecticidal activity by facilit...
17336267 - Dynamic interplay between the neutral glycosphingolipid cd77/gb3 and the therapeutic an...
7097007 - Development and application of an efficient procedure for converting mouse igm into sma...
8754847 - In vivo genomic footprinting of thyroid hormone-responsive genes in pituitary tumor cel...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  115     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-26     Completed Date:  2002-07-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  817-26     Citation Subset:  IM    
Affiliation:
Max-Planck-Institute of Biophysical Chemistry, Department of Neurobiology, Göttingen, Germany. imajoul@gwdg.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle*
Cells, Cultured
Cercopithecus aethiops
G(M1) Ganglioside / biosynthesis,  metabolism*
G0 Phase
G1 Phase
G2 Phase
Hippocampus / cytology
Mice
Neurons / metabolism
PC12 Cells
Rats
Receptors, Cell Surface / biosynthesis,  metabolism*
Telophase
Trihexosylceramides / biosynthesis,  metabolism*
Vero Cells
Chemical
Reg. No./Substance:
0/Receptors, Cell Surface; 0/Trihexosylceramides; 0/choleragen receptor; 37758-47-7/G(M1) Ganglioside; 71965-57-6/globotriaosylceramide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Mutational analysis of the variant surface glycoprotein GPI-anchor signal sequence in Trypanosoma br...
Next Document:  Identification and characterization of a novel human plant pathogenesis-related protein that localiz...