Document Detail

Differential expression of receptors for Shiga and Cholera toxin is regulated by the cell cycle.
MedLine Citation:
PMID:  11865037     Owner:  NLM     Status:  MEDLINE    
Cholera and Shiga toxin bind to the cell surface via glycolipid receptors GM1 and Gb3, respectively. Surprisingly, the majority of Vero cells from a non-synchronized population bind either Cholera or Shiga toxin but not both toxins. The hypothesis that the differential expression of toxin receptors is regulated by the cell cycle was tested. We find that Cholera toxin binds preferentially in G0/G1, with little binding through S-phase to telophase, whereas Shiga toxin binds maximally through G2 to telophase but does not bind during G0/G1 and S-phase. The changes result from the corresponding changes in Gb3 and GM1 synthesis, not from variations of receptor transport to the cell surface. The changes do not reflect competition of Gb3 and GM1 synthesis for lactosylceramide. Cells as diverse as Vero cells, PC12 cells and astrocytes show the same cell-cycle-dependent regulation of glycosphingolipid receptors, suggesting that this novel phenomenon is based on a conserved regulatory mechanism.
Irina Majoul; Tobias Schmidt; Maria Pomasanova; Evgenia Boutkevich; Yuri Kozlov; Hans-Dieter Söling
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  115     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-26     Completed Date:  2002-07-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  817-26     Citation Subset:  IM    
Max-Planck-Institute of Biophysical Chemistry, Department of Neurobiology, Göttingen, Germany.
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MeSH Terms
Cell Cycle*
Cells, Cultured
Cercopithecus aethiops
G(M1) Ganglioside / biosynthesis,  metabolism*
G0 Phase
G1 Phase
G2 Phase
Hippocampus / cytology
Neurons / metabolism
PC12 Cells
Receptors, Cell Surface / biosynthesis,  metabolism*
Trihexosylceramides / biosynthesis,  metabolism*
Vero Cells
Reg. No./Substance:
0/Receptors, Cell Surface; 0/Trihexosylceramides; 0/choleragen receptor; 37758-47-7/G(M1) Ganglioside; 71965-57-6/globotriaosylceramide

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