Document Detail


Differential expression profiles of the Hedgehog signaling pathway between microsatellite-stable and microsatellite-unstable colorectal cancers.
MedLine Citation:
PMID:  21725600     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study aimed to investigate the expression of the Hedgehog (Hh) signaling pathway between microsatellite-unstable (MSI) and microsatellite-unstable (MSS) colorectal cancers (CRCs). A total of 61 samples of CRC tissue and corresponding blood samples were obtained from the surgical department of our hospital. The tissue samples were examined by immunohistochemistry using antibodies against Sonic Hh (SHH), Pathed (PTCH) and Gli1, and evaluated independently for protein expression by two pathologists blinded to clinical outcome. Based on the immunohistochemistry results, SHH and PTCH expression varied in terms of histological type. In mucinous adenocarcinoma (MA) Hedgehog signaling was not highly expressed. There were more significant differences in the expression of SHH and PTCH (P<0.05), compared with Gli1. Moreover, significant differences were found in the expression of SHH, Gli1 and PTCH between the MSI and MSS groups (P<0.05). Hedgehog signals were more frequently expressed in the MSI group compared with the MSS group. In conclusion, this study indicates that the expression of the Hh signaling pathway may play a significant role in MSI in colorectal cancer.
Authors:
Xuguang Hu; Daili Lai; Wei Chen; Shuming Zi; Jinming Li; Peng Du; Zhongchuan Wang; Shiyong Huang; Cui Long
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-07-01
Journal Detail:
Title:  Molecular medicine reports     Volume:  4     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:    2011 Sep-Oct
Date Detail:
Created Date:  2011-07-13     Completed Date:  2011-11-01     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  873-7     Citation Subset:  IM    
Affiliation:
Department of Colorectal Surgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Colorectal Neoplasms / genetics*,  pathology
Female
Gene Expression Profiling*
Hedgehog Proteins / metabolism*
Humans
Immunohistochemistry
Male
Microsatellite Instability*
Microsatellite Repeats / genetics*
Middle Aged
Neoplasm Staging
Receptors, Cell Surface / metabolism
Signal Transduction*
Transcription Factors / metabolism
Chemical
Reg. No./Substance:
0/GLI1 protein, human; 0/Hedgehog Proteins; 0/Receptors, Cell Surface; 0/SHH protein, human; 0/Transcription Factors; 0/patched receptors

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