| Differential expression of peroxisome proliferator activated receptor gamma and cyclin D1 does not affect proliferation of asthma- and non-asthma-derived airway smooth muscle cells. | |
| | |
MedLine Citation:
|
PMID: 20070588 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PPARgamma levels in asthma- and non-asthma-derived airway smooth muscle cells and PPARgamma activation-induced cell proliferation were investigated. In the presence of FBS, PPARgamma levels were higher in subconfluent asthma-derived cells but lower in confluent cells compared with non-asthma-derived. However, PPARgamma activation did not alter cell proliferation. BACKGROUND AND OBJECTIVE: Airway remodelling involves thickening of the airway smooth muscle (ASM) bulk. Proliferation of asthma-derived ASM cells is increased in vitro, but underlying mechanisms remain unknown. Peroxisome proliferators activated receptor-gamma (PPARgamma) regulates the cell cycle. It is suggested that PPARgamma agonists have anti-inflammatory effects, which may be valuable in the treatment of asthma, but information regarding their antiproliferative properties in ASM is lacking. Although corticosteroids reduce airway inflammation, in vitro they inhibit proliferation in only non-asthma ASM cells by reducing cyclin D1. We therefore investigated the effects of mitogenic stimulation (foetal bovine serum (FBS)), and a PPARgamma ligand (ciglitazone), on PPARgamma and cyclin D1 expression and proliferation of ASM cells. In addition, we examined the effects of ciglitazone on ASM cell proliferation. METHODS: We assessed PPARgamma and cyclin D1 mRNA and protein levels using quantitative PCR and immunoblotting. Cell proliferation was assessed using bromodeoxyuridine uptake. RESULTS: In the presence of 5% FBS, PPARgamma and cyclin D1 expression decreased over time in non-asthmatic cells but increased in asthmatic cells (compared with sub-confluent cells). FBS-induced proliferation of asthmatic cells increased at all time points, but occurred only at day 7 with non-asthmatic cells (compared with unstimulated time-matched control). Ciglitazone increased PPARgamma expression in both groups, but did not alter cell proliferation, while fluticasone increased PPARgamma protein only in asthmatic cells. CONCLUSIONS: Although in the presence of a mitogenic stimulus, PPARgamma was differentially expressed in asthma- and non-asthma-derived ASM; its expression was not related to the increased proliferation observed in asthmatic ASM. |
| | |
Authors:
|
Justine Y Lau; Brian G Oliver; Lyn M Moir; Judith L Black; Janette K Burgess |
Related Documents
:
|
18283098 - Alcohol exposure alters cell cycle and apoptotic events during early neurulation. 18073448 - Cell cycle arrest of stamen initials in maize sex determination. 11464278 - The periodic down regulation of cyclin e gene expression from exit of mitosis to end of... 12119358 - Cytoplasmic p21(cip1/waf1) regulates neurite remodeling by inhibiting rho-kinase activity. 8185588 - Cellular localization of high-molecular-mass heat shock proteins in murine cells. 10852468 - Differential activity of the cytochrome p450 17alpha-hydroxylase and steroidogenic acut... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-11 |
Journal Detail:
|
Title: Respirology (Carlton, Vic.) Volume: 15 ISSN: 1440-1843 ISO Abbreviation: Respirology Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2010-03-04 Completed Date: 2010-06-21 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9616368 Medline TA: Respirology Country: Australia |
Other Details:
|
Languages: eng Pagination: 303-12 Citation Subset: IM |
Affiliation:
|
Cooperative Research Centre for Asthma and Airways, The University of Sydney, Sydney, New South Wales, Australia. jlau@med.usyd.edu.au |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Adult Aged Aged, 80 and over Androstadienes / pharmacology Asthma / metabolism*, pathology* Bronchi / pathology Bronchodilator Agents / pharmacology Cell Proliferation* / drug effects Cells, Cultured Cyclin D1 / metabolism* Female Humans Male Middle Aged Mitogens / pharmacology Myocytes, Smooth Muscle / drug effects, metabolism*, pathology* PPAR gamma / metabolism* RNA, Messenger / metabolism Thiazolidinediones / pharmacology Young Adult |
| Chemical | |
Reg. No./Substance:
|
0/Androstadienes; 0/Bronchodilator Agents; 0/Mitogens; 0/PPAR gamma; 0/RNA, Messenger; 0/Thiazolidinediones; 136601-57-5/Cyclin D1; 74772-77-3/ciglitazone; 90566-53-3/fluticasone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Seasonal variation in the incidence of spontaneous pneumothorax and its association with climate: A ...
Next Document: Productive cough is an independent risk factor for the development of COPD in former smokers.