Document Detail

Differential expression of p63 isotypes (DeltaN and TA) in salivary gland neoplasms: biological and diagnostic implications.
MedLine Citation:
PMID:  16084953     Owner:  NLM     Status:  MEDLINE    
To determine the association between the expression of p63 gene isoforms (TA and DeltaN) and salivary gland tumorigenesis, we performed reverse transcription-polymerase chain reaction analysis of these markers in 71 benign and malignant salivary gland neoplasms. The results were correlated with the expression of Notch ligand JAG1 gene and the clinicopathologic features and the full-length p63 protein expression by immunohistochemistry. Both p63 isoforms were either negative or weakly expressed in normal salivary gland tissues. TAp63 was highly expressed in most benign tumors and was either negative or weakly positive in most carcinomas. Conversely, DeltaNp63 was negative or faintly positive in most benign neoplasms and was highly expressed in adenoid cystic, mucoepidermoid, and myoepithelial carcinomas. Immunohistochemical analysis using anti-full-length p63 protein showed ubiquitous nuclear staining in basal and myoepithelial cells in both benign and malignant neoplasms. JAG1 was expressed in most benign and malignant tumors and did not correlate with p63 isoforms expression. We conclude that (1) p63 isoforms are differentially expressed in most benign and malignant tumors and may play distinct biological roles in certain salivary gland neoplasms; (2) p63 immunostaining do not correlate with the isoforms expression; and (3) isoform-specific antibodies are required for better cellular localization and biological correlations.
Shin-Ichiro Maruya; Merrill S Kies; Michelle Williams; Jeffery N Myers; Randal S Weber; John G Batsakis; Adel K El-Naggar
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Human pathology     Volume:  36     ISSN:  0046-8177     ISO Abbreviation:  Hum. Pathol.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-08-08     Completed Date:  2005-09-27     Revised Date:  2008-06-27    
Medline Journal Info:
Nlm Unique ID:  9421547     Medline TA:  Hum Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  821-7     Citation Subset:  IM    
Department of Pathology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
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MeSH Terms
Adenocarcinoma / genetics,  metabolism*,  pathology
Adenolymphoma / genetics,  metabolism*,  pathology
Adenoma, Pleomorphic / genetics,  metabolism*,  pathology
Calcium-Binding Proteins
DNA Primers / chemistry
DNA-Binding Proteins
Gene Expression Regulation, Neoplastic
Genes, Tumor Suppressor*
Immunoenzyme Techniques
Intercellular Signaling Peptides and Proteins
Membrane Proteins / genetics,  metabolism
Phosphoproteins / genetics,  metabolism*
Protein Isoforms
RNA, Messenger / metabolism
RNA, Neoplasm / genetics
Reverse Transcriptase Polymerase Chain Reaction
Salivary Gland Neoplasms / genetics,  metabolism*,  pathology
Salivary Glands / metabolism,  pathology
Trans-Activators / genetics,  metabolism*
Tumor Suppressor Proteins
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/DNA Primers; 0/DNA-Binding Proteins; 0/Intercellular Signaling Peptides and Proteins; 0/Membrane Proteins; 0/Phosphoproteins; 0/Protein Isoforms; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/TP63 protein, human; 0/Trans-Activators; 0/Tumor Suppressor Proteins; 134324-36-0/Serrate proteins

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