Document Detail


Differential expression of nucleophosmin and stathmin in human T lymphoblastic cell lines, CCRF-CEM and JURKAT analyzed by two-dimensional gel electrophoresis.
MedLine Citation:
PMID:  8529626     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two-dimensional gel electrophoresis was used to study the expression of intracellular proteins in adherent cells of human T lymphoblastic cell line, CCRF-CEM. The adherent cells grown in monolayer on a culture plate decreased the amount of proteins of M(r) 37,000 and pI 4.7-4.9, and of 17,000 and pI 5.7. The proteins were identified to be nucleophosmin for the 37,000 protein and stathmin for the 17,000 protein by microsequencing their CNBr fragments. The amount of proteins was increased in CCRF-CEM cells grown in floating mass to a comparable level of JURKAT cells which grew in floating mass throughout the culture. The adherent cells decreased their growth rate as compared with the cells in the floating mass. These results suggest that the adhesion of human T lymphoblastic cells modulates their morphology and proliferation via a concomitant decrease in the amount of nucleophosmin and stathmin.
Authors:
K Nakamura; M Fujimoto; T Tanaka; Y Fujikura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  16     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1996-01-29     Completed Date:  1996-01-29     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  1530-5     Citation Subset:  IM    
Affiliation:
First Department of Biochemistry, Yamaguchi University School of Medicine, Ube, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cattle
Cell Adhesion
Cell Division
Cell Line
Culture Media
Electrophoresis, Gel, Two-Dimensional / methods*
Electrophoresis, Polyacrylamide Gel
Fetal Blood
Humans
Isoelectric Focusing
Isoelectric Point
Microtubule Proteins*
Molecular Sequence Data
Molecular Weight
Nuclear Proteins / analysis*,  chemistry
Phosphoproteins / analysis*,  chemistry
Sequence Analysis
Stathmin
T-Lymphocytes / chemistry*,  cytology
Chemical
Reg. No./Substance:
0/Culture Media; 0/Microtubule Proteins; 0/Nuclear Proteins; 0/Phosphoproteins; 0/STMN1 protein, human; 0/Stathmin; 117896-08-9/nucleophosmin

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