Document Detail


Differential expression of miRNA-146a-regulated inflammatory genes in human primary neural, astroglial and microglial cells.
MedLine Citation:
PMID:  21640790     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MicroRNA-146a (miRNA-146a) is an inducible, 22 nucleotide, small RNA over-expressed in Alzheimer's disease (AD) brain. Up-regulated miRNA-146a targets several inflammation-related and membrane-associated messenger RNAs (mRNAs), including those encoding complement factor-H (CFH) and the interleukin-1 receptor associated kinase-1 (IRAK-1), resulting in significant decreases in their expression (p<0.05, ANOVA). In this study we assayed miRNA-146a, CFH, IRAK-1 and tetraspanin-12 (TSPAN12), abundances in primary human neuronal-glial (HNG) co-cultures, in human astroglial (HAG) and microglial (HMG) cells stressed with Aβ42 peptide and tumor necrosis factor alpha (TNFα). The results indicate a consistent inverse relationship between miRNA-146a and CFH, IRAK-1 and TSPAN12 expression levels, and indicate that HNG, HAG and HMG cell types each respond differently to Aβ42-peptide+TNFα-triggered stress. While the strongest miRNA-146a-IRAK-1 response was found in HAG cells, the largest miRNA-146a-TSPAN12 response was found in HNG cells, and the most significant miRNA-146a-CFH changes were found in HMG cells, the 'resident scavenging macrophages' of the brain.
Authors:
Yuan Yuan Li; Jian Guo Cui; Prerna Dua; Aileen I Pogue; Surjyadipta Bhattacharjee; Walter J Lukiw
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-05-26
Journal Detail:
Title:  Neuroscience letters     Volume:  499     ISSN:  1872-7972     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-27     Completed Date:  2012-05-14     Revised Date:  2013-07-21    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  109-13     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
LSU Neuroscience Center, Department of Ophthalmology, Louisiana State University Health Science Center, New Orleans, LA 70112, USA.
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MeSH Terms
Descriptor/Qualifier:
Amyloid beta-Peptides / physiology
Astrocytes / pathology,  physiology*
Cells, Cultured
Coculture Techniques
Complement Factor H / antagonists & inhibitors,  genetics
Gene Expression Regulation*
Humans
Inflammation Mediators / physiology*
MicroRNAs / biosynthesis*,  genetics,  physiology
Microglia / pathology,  physiology*
Neurons / pathology,  physiology*
Peptide Fragments / physiology
Tumor Necrosis Factor-alpha / physiology
Grant Support
ID/Acronym/Agency:
AG038834/AG/NIA NIH HHS; R01 AG038834/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Amyloid beta-Peptides; 0/Inflammation Mediators; 0/MIRN146 microRNA, human; 0/MicroRNAs; 0/Peptide Fragments; 0/Tumor Necrosis Factor-alpha; 0/amyloid beta-protein (1-42); 80295-65-4/Complement Factor H
Comments/Corrections
Erratum In:
Neurosci Lett. 2012 Jun 27;520(1):126

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