Document Detail


Differential expression of matrix metalloproteinases and tissue-derived inhibitors of metalloproteinase in fetal and adult skins.
MedLine Citation:
PMID:  17409012     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Matrix metalloproteinases and their tissue-derived inhibitors determine the architecture of the extracellular matrix. In early gestation, the amount and organization of extracellular matrix may be associated with scarless repair of fetal skin wounds. To elucidate the part of the mechanism(s) underlying the phenotypic transition from scarless to scar-forming healing observed during fetal gestation, the ontogeny of matrix metalloproteinase-2, -9, -14 and their tissue inhibitors was characterized in non-wounded fetal human skin with different gestational ages from 13 to 33 weeks and adult skin using reverse transcriptase-polymerase chain reaction, immunohistochemical staining and western blot protocols. We showed that the levels of gene expressions for matrix metalloproteinase-2, -9, -14 and their endogenous inhibitors were significantly more in late gestational and adult skins than that in early gestational skin. Similar results were noted in terms of protein contents of these enzymes and inhibitors in fetal and adult skins. We concluded that the endogenous matrix metalloproteinase-2, -9, -14 and their endogenous inhibitors might be involved in skin development and in maintenance of cutaneous structure and function. Lower protein contents of tissue-derived inhibitor-1, -2 in early gestational skin might provide a predominantly antiscarring signal while higher protein expression of these two inhibitors might be associated with scar-forming healing in late gestational and adult skins.
Authors:
Wei Chen; Xiaobing Fu; Shili Ge; Tongzhu Sun; Zhiyong Sheng
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-02
Journal Detail:
Title:  The international journal of biochemistry & cell biology     Volume:  39     ISSN:  1357-2725     ISO Abbreviation:  Int. J. Biochem. Cell Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-04-20     Completed Date:  2007-08-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508482     Medline TA:  Int J Biochem Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  997-1005     Citation Subset:  IM    
Affiliation:
Key Research Laboratory of Wound Repair, The First Affiliated Hospital, 301th Hospital of Beijing, 100037 Beijing, China. chenwei304@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Blotting, Western
Female
Fetus / metabolism*
Gene Expression Regulation, Developmental
Gestational Age
Humans
Immunohistochemistry
Isoenzymes / genetics,  metabolism
Matrix Metalloproteinase 14 / genetics,  metabolism
Matrix Metalloproteinase 2 / genetics,  metabolism
Matrix Metalloproteinase 9 / genetics,  metabolism
Matrix Metalloproteinases / genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Skin / embryology,  metabolism*
Tissue Inhibitor of Metalloproteinase-1 / genetics,  metabolism*
Tissue Inhibitor of Metalloproteinase-2 / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/RNA, Messenger; 0/Tissue Inhibitor of Metalloproteinase-1; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9; EC 3.4.24.80/Matrix Metalloproteinase 14

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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