Document Detail

Differential expression of matrix metalloproteinase-2 by fibroblasts in co-cultures with keratinocytes, basal cell carcinoma and melanoma.
MedLine Citation:
PMID:  16958805     Owner:  NLM     Status:  MEDLINE    
To address whether matrix metalloproteinase (MMP)-2 expression by mesenchymal fibroblasts may be differentially modulated by interactions with normal or malignant epidermal cells, we set up a fibroblast co-culture model separately with keratinocytes, basal cell carcinoma cells and melanoma cells. Real-time reverse transcription polymerase chain reaction (RT-PCR) and gelatin zymography were used to detect and quantify the expression of MMP-2 by these different kinds of cells. In independent cultures, MMP-2 was highly expressed by fibroblasts and melanoma cells constitutively, but barely expressed by keratinocytes and basal cell carcinoma (BCC) cells. MMP-2 expression by fibroblasts was downregulated in co-cultures with keratinocytes or BCC cells, but not with melanoma cells. These results indicate that epidermal-mesenchymal interactions are important modulators for MMP-2 expression by cutaneous cells and suggest a host-defense mechanism against the tumor progression of BCC.
Gwo-Shing Chen; Mei-Pao Lu; Meng-Tse Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of dermatology     Volume:  33     ISSN:  0385-2407     ISO Abbreviation:  J. Dermatol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-08     Completed Date:  2006-11-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600545     Medline TA:  J Dermatol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  609-15     Citation Subset:  IM    
Department of Dermatology, Kaohsiung Medical University, Kaohsiung, Taiwan.
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MeSH Terms
Carcinoma, Basal Cell / enzymology*
Cell Culture Techniques
Cell Line, Tumor
Coculture Techniques
Fibroblasts / physiology*
Keratinocytes / physiology*
Matrix Metalloproteinase 2 / metabolism*
Melanoma / enzymology*
Skin Neoplasms / enzymology*
Reg. No./Substance:
EC Metalloproteinase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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