| Differential expression of isomorphic HLA-DR beta genes is not a sole function of transcription. | |
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MedLine Citation:
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PMID: 8891734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells expressing HLA DR7 contain two functional DR beta genes, DRB1 and DRB4, whose mRNA is present at different levels. We examined whether the mRNA levels are due to differential transcriptional regulation or post-transcriptional regulation. As part of this analysis, a novel series of upstream elements was identified. Analysis of the proximal promoter activity, using a transient expression system, showed that the isomorphic promoter activities of B1 and B4 are about equal. RNase protection analysis of steady-state pre-mRNA and mRNA levels shows that the DR7 B1 pre-mRNA levels are 3 to 4 fold higher than B4 pre-mRNA levels. However, the B1 mRNA levels are increased seven fold relative to the B4 mRNA levels. The disproportionate increase of the mRNA levels relative to the corresponding pre-mRNA levels indicates that regulation also occurs at the post-transcriptional level. |
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Authors:
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M P Leen; J Gorski |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Human immunology Volume: 50 ISSN: 0198-8859 ISO Abbreviation: Hum. Immunol. Publication Date: 1996 Oct |
Date Detail:
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Created Date: 1997-02-27 Completed Date: 1997-02-27 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8010936 Medline TA: Hum Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 111-20 Citation Subset: IM |
Affiliation:
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Blood Research Institute of the Blood Center of S.E. Wisconsin, Department of Immunogenetics, Milwaukee, 53201, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Gene Expression Regulation / genetics* HLA-DR Antigens / biosynthesis*, classification, genetics* Humans Molecular Sequence Data Promoter Regions, Genetic / genetics RNA, Messenger / analysis Transcription, Genetic / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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P0 HL44612/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/HLA-DR Antigens; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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