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Differential expression of iron metabolism proteins in diabetic and diabetic iron-supplemented rat liver.
MedLine Citation:
PMID:  22170771     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Diabetes mellitus is associated with altered iron homeostasis that can potentially effect reactive oxygen species generation and contribute to diabetes-related complications. We investigated, by quantitative polymerase chain reaction, whether the expression of liver hepcidin, ferritin, and TfR-1 is altered in diabetes. Rats in the control (C) group received a standard diet; control iron (CI) group received a standard diet supplemented with iron; diabetic (D) group received an injection of streptozotocin; and diabetic iron (DI) group received streptozotocin and the diet with iron. Animals of the D group showed higher levels of serum iron, increased concentration of carbonyl protein, and a decrease in antioxidant status. Group D rats showed increased hepatic expression of Trf-1 compared to the other groups. Iron supplementation reversed this increase. Hepcidin mRNA was 81% higher in DI than in C and CI rats. The results suggest that diabetes, with or without excess iron, can cause perturbations in iron status, hepcidin and Trf-1 expression. © 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 00:1-7 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20418.
Authors:
Maísa Silva; Cintia Lopes de Brito Magalhães; Riva de Paula Oliveira; Marcelo Eustáquio Silva; Maria Lucia Pedrosa
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-14
Journal Detail:
Title:  Journal of biochemical and molecular toxicology     Volume:  -     ISSN:  1099-0461     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9717231     Medline TA:  J Biochem Mol Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Wiley Periodicals, Inc.
Affiliation:
Programa de Pós graduação em Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil. maisasilv@yahoo.com.br.
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