| Differential expression of human topoisomerase IIIalpha during the cell cycle progression in HL-60 leukemia cells and human peripheral blood lymphocytes. | |
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MedLine Citation:
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PMID: 10739669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human topoisomerase IIIalpha (huTop IIIalpha) has been demonstrated to belong to type IA subfamily. In this study, we found that huTop IIIalpha expressed constitutively and remained at high levels throughout the cell cycle in HL-60 cells when compared to the cell-cycle-dependent expression of huTop IIIalpha in phytohemagglutinin-activated peripheral blood lymphocytes. During the cell cycle progression, this protein remained accentuated in the nucleolus without significant translocation from the nucleolus to the nucleoplasm. In addition, during the course of granulocytic maturation in DMSO-treated HL-60 cells, huTop IIIalpha levels decreased when cells stopped proliferation and nucleoli diminished in size. However, its level remained unchanged during the course of monocytic maturation of vitamin D(3)-treated HL-60 cells which still retained its proliferative capacity and did not change the size of the nucleolus. The data suggested that huTop IIIalpha is involved in rDNA metabolism, such as rDNA transcription. Its cellular level appeared to be under control during the cell cycle progression of normal lymphocytes, but was found to be deregulated in HL-60 cells which may be associated with the tumor transformed cell phenotypes. |
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Authors:
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C W Lin; Z Darzynkiewicz; X Li; F Traganos; E Bedner; Y C Tse-Dinh |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental cell research Volume: 256 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-05-12 Completed Date: 2000-05-12 Revised Date: 2010-12-03 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 225-36 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Academic Press. |
Affiliation:
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Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Antibodies Cell Cycle / physiology* Cell Differentiation / drug effects Cell Nucleolus / enzymology Cell Nucleus / metabolism Cells, Cultured DNA Topoisomerases, Type I / analysis, metabolism* Dimethyl Sulfoxide / pharmacology Granulocytes / enzymology HL-60 Cells Humans Lymphocyte Activation Lymphocytes / cytology*, enzymology* Molecular Sequence Data Peptide Fragments / chemistry, immunology Photolysis |
| Grant Support | |
ID/Acronym/Agency:
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CA-R01-28704/CA/NCI NIH HHS; GM-R01-54226/GM/NIGMS NIH HHS; R01 CA028704-21/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Peptide Fragments; 67-68-5/Dimethyl Sulfoxide; EC 5.99.1.-/DNA topoisomerase III; EC 5.99.1.2/DNA Topoisomerases, Type I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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