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Differential expression of the human ST5 gene in HeLa-fibroblast hybrid cell lines mediated by YY1: evidence that YY1 plays a part in tumor suppression.
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MedLine Citation:
PMID:  8972856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Through a mutational analysis of a differentially regulated enhancer, we present evidence that supports a role for the transcription factor YY1 in tumor suppression in HeLa/fibroblast somatic cell hybrids. The human ST5 gene was previously shown to be expressed as three RNA species, 4.6, 3.1 and 2.8 kb in length. Whereas the two larger species are expressed at similar levels in all cell lines examined, the 2.8 kb mRNA is expressed specifically in non-tumorigenic hybrids. In this study, the basis for the differential expression of this mRNA species was investigated. The message was shown to originate from a promoter located within an intron of the ST5 gene. An enhancer located approximaely 1500 nt upstream of the start site was required for cell type specific expression. Mutational analysis of this enhancer revealed an AP1 site and five YY1 sites which were necessary for full enhancer activity. Levels of YY1 DNA binding activity were found to be as much as 6-fold higher in the non-tumorigenic cells relative to the tumorigenic cells, while AP1 activity was similar in both cell types. These results suggest that a signaling pathway targeting YY1 may play an important role in tumor suppression in HeLa-fibroblast hybrids.
Authors:
J H Lichy; M Majidi; J Elbaum; M M Tsai
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  24     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-01-30     Completed Date:  1997-01-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4700-8     Citation Subset:  IM    
Affiliation:
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, DC 20306-6000, USA. lichy@email.afip.osd.mil
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U15131;  U15132;  U15779;  U15780
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Binding Sites
Cell Line
DNA-Binding Proteins / metabolism*
Enhancer Elements, Genetic
Erythroid-Specific DNA-Binding Factors
Fibroblasts*
Gene Expression*
Hela Cells*
Humans
Hybrid Cells / metabolism*
Introns
Molecular Sequence Data
Mutagenesis
Point Mutation
Promoter Regions, Genetic
RNA / chemistry,  metabolism
RNA, Messenger / genetics*,  metabolism
Transcription Factor AP-1 / metabolism
Transcription Factors / metabolism*
YY1 Transcription Factor
Grant Support
ID/Acronym/Agency:
R01CA-64114/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Erythroid-Specific DNA-Binding Factors; 0/RNA, Messenger; 0/Transcription Factor AP-1; 0/Transcription Factors; 0/YY1 Transcription Factor; 0/YY1 protein, human; 63231-63-0/RNA
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 1 Month: 12 Year: 1996
Volume: 24 Issue: 23
First Page: 4700 Last Page: 4708
ID: 146314
PubMed Id: 8972856
Publisher Item Identifier: 6e0422

Differential expression of the human ST5 gene in HeLa-fibroblast hybrid cell lines mediated by YY1: evidence that YY1 plays a part in tumor suppression.
J H Lichy
M Majidi
J Elbaum
M M Tsai
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, DC 20306-6000, USA. lichy@email.afip.osd.mil


Article Categories:
  • Research Article


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