Document Detail

Differential expression of the glycosylated forms of MUC1 during lung development.
MedLine Citation:
PMID:  17664159     Owner:  NLM     Status:  MEDLINE    
Human MUC1 mucin is a high-molecular weight transmembrane glycoprotein expressed on the apical surface of the simple epithelia of many different tissues. Previous investigations suggest the involvement of MUC1 in epithelial cytodifferentiation and glandular morphogenesis. However, the role of MUC1 in the development of the fetal respiratory tracts has so far been poorly investigated. To obtain more information on the roles of MUC1 during fetal lung development, we examined the expression and distribution of MUC1 by immunohistochemical staining of postmortem lung specimens from fetuses and neonates of various gestational ages. Three monoclonal antibodies, HMFG1, HMFG2, and anti-KL-6, which bind different glycosylation variants, were used. Each monoclonal antibody has been shown to recognize heavily-glycosylated MUC1, sparsely-glycosylated MUC1, and sialylated carbohydrate side chains of MUC1, respectively. At 13 weeks of gestation, the terminal respiratory tracts were diffusely stained with HFMG1 and anti-KL-6. Sparsely-glycosylated MUC1, as recognized by HMFG2, was detected only in the distal portions of the terminal bronchioles that divided into respiratory bronchioles. As such development continued, MUC1, recognized by HMFG1 and anti-KL-6, was detected throughout the bronchioles and terminal sacs, although HMFG1 immunoreactivity decreased in intensity towards the terminal sacs. Sparsely-glycosylated MUC1, as recognized by HMFG2, was mainly observed in the terminal portions. In the adult lungs, both the alveolar spaces and the respiratory bronchioles stained with HFMG1 and anti-KL-6. However, the distribution of sparsely-glycosylated MUC1 was limited in the alveolar epithelial cells. Our investigation demonstrated that variants of MUC1 were expressed in the fetal respiratory tracts as early as 13 weeks of gestation, and its expression persisted even after lung maturation. The precise roles of MUC1 were not determined in the present study; however, different glycosylation variants of MUC1 may be associated with the development of different regions of the terminal respiratory tract.
J Sakurai; N Hattori; M Nakajima; T Moriya; T Suzuki; A Yokoyama; N Kohno
Related Documents :
3337839 - Postnatal transformations of alveolar surfactant in the rabbit: changes in pool size, p...
9511189 - Fetal lung volumetry by three-dimensional ultrasound.
8889629 - Ultrasonic fetal and placental tissue characterization and lung maturity.
8531149 - A comparison of three wetting agents used to facilitate the pouring of dies.
6198659 - Evidence for a clonal model for hemoglobin switching.
3456149 - Prostaglandin concentrations in ovine maternal and fetal tissues at late gestation.
7193959 - Estimation of gestosis of pregnancy (eph-gestosis), relationship between fetal developm...
1879389 - Effects of reviewing risk-relevant behavior on perceived vulnerability among women mari...
535879 - Total cortisol and l/s-ratio in amniotic fluid in late pregnancies complicated by diabe...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of histochemistry : EJH     Volume:  51     ISSN:  1121-760X     ISO Abbreviation:  Eur J Histochem     Publication Date:    2007 Apr-Jun
Date Detail:
Created Date:  2007-07-31     Completed Date:  2007-12-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9207930     Medline TA:  Eur J Histochem     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  95-102     Citation Subset:  IM    
Department of Molecular and Internal Medicine, Graduate School of Biochemical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Fetus / embryology,  metabolism
Gestational Age
Infant, Newborn
Lung / embryology*,  growth & development,  metabolism*
Mucin-1 / chemistry,  metabolism*
Reg. No./Substance:
0/MUC1 protein, human; 0/Mucin-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Possible involvement of DNA strand breaks in regulation of cell differentiation.
Next Document:  Effect of recombinant human activated protein C on apoptosis-related proteins.