| Differential expression of glycoproteins containing alpha-D-galactosyl groups on normal human breast epithelial cells and MCF-7 human breast carcinoma cells. | |
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MedLine Citation:
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PMID: 1913627 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell surface glycoproteins were isolated from the lysates of 125I-labeled normal human mammary epithelial cells (NHMEC) and from the human breast carcinoma cell line MCF-7, of blood-group O phenotype, by affinity chromatography on Griffonia simplicifolia I lectin-Sepharose. Specific elution of glycoproteins from the column with methyl alpha-D-galactoside suggests the presence of alpha-D-galactosyl groups on these moieties. SDS-PAGE analysis of isolated glycoproteins revealed both quantitative and qualitative differences between glycoproteins from normal and malignant cells. Three major glycoproteins of Mr 180 kDa, 85 kDa and the 44 kDa were obtained from MCF-7 cells. The 180-kDa glycoprotein was absent in NHMEC and the 44-kDa glycoprotein was very weakly expressed in these cells. The only glycoprotein which was found in almost equal amount in the lysate from both normal and malignant cells was the 85-kDa glycoprotein. These results indicate differences between normal human mammary epithelial cells and one kind of malignant human mammary epithelial cells, in the expression of glycoproteins containing alpha-D-galactosyl groups, irrespective of blood-group phenotype; they also demonstrate that alpha-D-galactosyl group are expressed in a very restrictive manner on the surface of this tumor cell line. |
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Authors:
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J Petryniak; J Varani; P R Ervin; I J Goldstein |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer letters Volume: 60 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1991-10-30 Completed Date: 1991-10-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 59-65 Citation Subset: IM |
Affiliation:
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Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109-0606. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Breast
/
chemistry*,
cytology Breast Neoplasms / chemistry* Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Epithelial Cells Epithelium / chemistry Galactose / analysis* Humans Membrane Glycoproteins / analysis* Neoplasm Proteins / analysis* Tumor Cells, Cultured / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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CA 20424/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Membrane Glycoproteins; 0/Neoplasm Proteins; 26566-61-0/Galactose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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