Document Detail

Differential expression of glucose transporters in rabbit placenta: effect of hypercholesterolemia in dams.
MedLine Citation:
PMID:  17135483     Owner:  NLM     Status:  MEDLINE    
Low birth weight is observed in rabbit offspring when maternal hypercholesterolemia is induced during gestation, but the related etiology is still unknown. Glucose is one of the most important substances during fetal development, and defect in glucose supply to fetus was related to pathophysiological mechanisms in intrauterine growth restriction. Thus, the aim of this work was to evaluate the impact of maternal hypercholesterolemia during rabbit gestation on the glucose metabolism and the routing of glucose transporters (SLC2 and SLC5 [previously known as GLUT and SGLT]) in placenta. In this study, maternal and offspring serum levels of glucose and insulin were evaluated for control and hypercholesterolemic groups, and the mRNA and protein expressions of placental SLCs were quantified by real-time RT-PCR and Western immunoblot, respectively. Our data demonstrate that maternal hypercholesterolemia during gestation: 1) induces offspring hypoglycemia; 2) does not modify the genetic and protein expressions of SLC2A1 and SLC2A4 (previously GLUT1 and GLUT4) in total placental extract; 3) downregulates the placental SLC5A1 (previously SGLT1) protein expression without affecting its mRNA levels; 4) impairs the translocation of SLC2A1 but not SLC2A4 from cytoplasmatic pool to the cell membrane surface. Then we assume that reduction of offspring birth weight in presence of maternal hypercholesterolemia may be related to the offspring's hypoglycemia and the reduction of the cell surface expression of placental SLC2A1.
Olha Kevorkova; Maude Ethier-Chiasson; Julie Lafond
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-29
Journal Detail:
Title:  Biology of reproduction     Volume:  76     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-21     Completed Date:  2007-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  487-95     Citation Subset:  IM    
Laboratoire de Physiologie Materno-Foetale, and Centre de Recherche BioMed, Université du Québec à Montréal, Montréal, Québec, Canada H3C 3P8.
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MeSH Terms
Animals, Newborn
Blood Glucose / metabolism
Body Weight
Gene Expression Regulation, Developmental
Glucose Transport Proteins, Facilitative / genetics,  metabolism*
Glucose Transporter Type 1 / genetics,  metabolism
Glucose Transporter Type 3 / genetics,  metabolism
Glucose Transporter Type 4 / genetics,  metabolism
Hypercholesterolemia / metabolism*
Insulin / blood
Organ Size
Placenta / physiology*
Pregnancy Complications / metabolism
Protein Transport
Sodium-Glucose Transporter 1 / genetics,  metabolism
Reg. No./Substance:
0/Blood Glucose; 0/Glucose Transport Proteins, Facilitative; 0/Glucose Transporter Type 1; 0/Glucose Transporter Type 3; 0/Glucose Transporter Type 4; 0/Sodium-Glucose Transporter 1; 11061-68-0/Insulin

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