Document Detail


Differential expression of galectin-1 and its interactions with cells and laminins in the intervertebral disc.
MedLine Citation:
PMID:  22692729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Galectin-1 (Gal-1), an endogenous β-galactoside-binding protein, binds to laminins, which are highly expressed in the nucleus pulposus (NP) of the intervertebral disc (IVD). The objective of this study is to evaluate the expression of Gal-1 protein in IVD tissues during aging and the effect of Gal-1 on IVD cell adhesion to laminins. Tissues from rat, porcine, and human (scoliosis or disc degeneration) IVDs were used to evaluate Gal-1 expression via immunostaining, RT-PCR, and Western blot analysis. Attachment of isolated IVD cells (porcine and human) on select laminin isoforms (LM-111 and LM-511) was compared with/without pre-incubation with exogenous Gal-1. A biotinylated Gal-1(B-Gal-1) was used to evaluate for binding to IVD cells and to select for IVD cells by magnetic activated cell sorting (MACS). NP cells expressed high levels of Gal-1 protein as compared to anulus fibrosus (AF) cells in immature tissues, while exogenous Gal-1 increased both NP and AF cell attachment to laminins and exhibited a similar binding to both cell types in vitro. With aging, Gal-1 levels in NP tissue appeared to decrease. In addition, incubation with B-Gal-1 was able to promote the retention of more than 50% of IVD cells via MACS. Our results provide new findings for the presence and functional role of Gal-1 within IVDs. Similar staining patterns for Gal-1 and LM-511 in IVD tissue suggest that Gal-1 may serve as an adhesion molecule to interact with both cells and laminins. This MACS protocol may be useful for selecting pure IVD cells from mixed cells of pathological tissue.
Authors:
Liufang Jing; Stephen So; Shaun W Lim; William J Richardson; Robert D Fitch; Lori A Setton; Jun Chen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-06-12
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  30     ISSN:  1554-527X     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-10-23     Completed Date:  2013-01-14     Revised Date:  2013-12-05    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1923-31     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Orthopaedic Research Society.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Animals
Biotinylation
Cell Adhesion
Cell Adhesion Molecules / metabolism
Cell Separation / methods
Child
Extracellular Matrix / metabolism
Galectin 1 / biosynthesis*
Gene Expression Regulation*
Humans
Intervertebral Disc / growth & development,  metabolism*
Laminin / metabolism*
Middle Aged
Models, Biological
Swine
Grant Support
ID/Acronym/Agency:
P01 AR050245/AR/NIAMS NIH HHS; P01AR050245/AR/NIAMS NIH HHS; R01 AR045644/AR/NIAMS NIH HHS; R01 AR047442/AR/NIAMS NIH HHS; R01 AR057410/AR/NIAMS NIH HHS; R01 EB002263/EB/NIBIB NIH HHS; R01AR047442/AR/NIAMS NIH HHS; R01AR057410/AR/NIAMS NIH HHS; R01EB002263/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Galectin 1; 0/Laminin
Comments/Corrections

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