| Differential expression and function of apoptosis-associated tyrosine kinase (AATYK) in the developing mouse brain. | |
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MedLine Citation:
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PMID: 12670708 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptosis-associated tyrosine kinase (AATYK) is a non-receptor type tyrosine kinase that is predominantly expressed in adult mouse brain. Although it is also expressed in developing brains, its expression pattern and physiological functions are unclear. In the present study, we analyzed expression profiles of AATYK in developing mouse brains and its functional role and subcellular localization in cultured cerebellar granule cells. Expression of AATYK mRNA and protein increased during postnatal brain development. Immunohistochemical analysis indicated that the protein was differentially expressed in postmitotic neurons within various brain areas including the olfactory bulb, cerebral cortex, hippocampus, thalamus, colliculus, cerebellum, and brain stem. Developmental increases in its expression were also observed in cultured cerebellar granule cells. AATYK protein was largely fractionated into the microsomal fraction and was immunocytochemically distributed in an ER-like meshwork of the granule cell soma, suggesting a possible association with the ER membrane. AATYK protein was also present in neurites. In immature granule cells, overexpression of wild-type AATYK promoted neurite outgrowth, whereas that of tyrosine kinase-defective mutant significantly inhibited it. These results suggest that, in addition to its role in cell death in mature neurons, AATYK has a unique role in promoting neurite extension through its tyrosine kinase activity in developing neurons. |
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Authors:
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Mineko Tomomura; Yuki Hasegawa; Tsutomu Hashikawa; Akito Tomomura; Michisuke Yuzaki; Teiichi Furuichi; Ryoji Yano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brain research. Molecular brain research Volume: 112 ISSN: 0169-328X ISO Abbreviation: Brain Res. Mol. Brain Res. Publication Date: 2003 Apr |
Date Detail:
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Created Date: 2003-04-02 Completed Date: 2003-07-31 Revised Date: 2012-06-04 |
Medline Journal Info:
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Nlm Unique ID: 8908640 Medline TA: Brain Res Mol Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 103-12 Citation Subset: IM |
Affiliation:
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Laboratory for Cellular Information Processing, Brain Science Institute (BSI), RIKEN, Wako, Japan. mtomomura@brain.riken.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Apoptosis / physiology* Apoptosis Regulatory Proteins Brain / cytology, enzymology*, growth & development* Cell Compartmentation / physiology* Cell Differentiation / physiology* Cells, Cultured Cerebellar Cortex / metabolism Endoplasmic Reticulum / metabolism Gene Expression Regulation, Enzymologic / genetics Immunohistochemistry Intracellular Membranes / metabolism Mice Mice, Inbred ICR Mutation / genetics Neurites / metabolism Neurons / cytology, enzymology* Protein-Tyrosine Kinases / deficiency*, genetics RNA, Messenger / metabolism Up-Regulation / genetics |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Regulatory Proteins; 0/RNA, Messenger; EC 2.7.10.1/Aatk protein, mouse; EC 2.7.10.1/Protein-Tyrosine Kinases |
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