Document Detail

Differential expression and function of apoptosis-associated tyrosine kinase (AATYK) in the developing mouse brain.
MedLine Citation:
PMID:  12670708     Owner:  NLM     Status:  MEDLINE    
Apoptosis-associated tyrosine kinase (AATYK) is a non-receptor type tyrosine kinase that is predominantly expressed in adult mouse brain. Although it is also expressed in developing brains, its expression pattern and physiological functions are unclear. In the present study, we analyzed expression profiles of AATYK in developing mouse brains and its functional role and subcellular localization in cultured cerebellar granule cells. Expression of AATYK mRNA and protein increased during postnatal brain development. Immunohistochemical analysis indicated that the protein was differentially expressed in postmitotic neurons within various brain areas including the olfactory bulb, cerebral cortex, hippocampus, thalamus, colliculus, cerebellum, and brain stem. Developmental increases in its expression were also observed in cultured cerebellar granule cells. AATYK protein was largely fractionated into the microsomal fraction and was immunocytochemically distributed in an ER-like meshwork of the granule cell soma, suggesting a possible association with the ER membrane. AATYK protein was also present in neurites. In immature granule cells, overexpression of wild-type AATYK promoted neurite outgrowth, whereas that of tyrosine kinase-defective mutant significantly inhibited it. These results suggest that, in addition to its role in cell death in mature neurons, AATYK has a unique role in promoting neurite extension through its tyrosine kinase activity in developing neurons.
Mineko Tomomura; Yuki Hasegawa; Tsutomu Hashikawa; Akito Tomomura; Michisuke Yuzaki; Teiichi Furuichi; Ryoji Yano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  112     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-02     Completed Date:  2003-07-31     Revised Date:  2012-06-04    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  103-12     Citation Subset:  IM    
Laboratory for Cellular Information Processing, Brain Science Institute (BSI), RIKEN, Wako, Japan.
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MeSH Terms
Animals, Newborn
Apoptosis / physiology*
Apoptosis Regulatory Proteins
Brain / cytology,  enzymology*,  growth & development*
Cell Compartmentation / physiology*
Cell Differentiation / physiology*
Cells, Cultured
Cerebellar Cortex / metabolism
Endoplasmic Reticulum / metabolism
Gene Expression Regulation, Enzymologic / genetics
Intracellular Membranes / metabolism
Mice, Inbred ICR
Mutation / genetics
Neurites / metabolism
Neurons / cytology,  enzymology*
Protein-Tyrosine Kinases / deficiency*,  genetics
RNA, Messenger / metabolism
Up-Regulation / genetics
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/RNA, Messenger; EC protein, mouse; EC Kinases

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