Document Detail

Differential expression of cyclin D1 in mantle cell lymphoma and other non-Hodgkin's lymphomas.
MedLine Citation:
PMID:  9846986     Owner:  NLM     Status:  MEDLINE    
Mantle-cell lymphomas are associated with a characteristic chromosomal translocation, t(11;14)(q13;q32). This translocation involves rearrangement of the bcl-1 proto-oncogene from chromosome 11 to the immunoglobulin heavy chain gene on chromosome 14, resulting in an overexpression of cyclin D1 mRNA (also known as bcl-1 and PRAD1). In the current study performed on paraffin-embedded tissue, cyclin D1 mRNA could be detected in 23 of 24 mantle-cell lymphomas by reverse transcription polymerase chain reaction (RT-PCR) whereas only 9 of 24 demonstrated a t(11;14) by PCR. However, we also found that cyclin D1 mRNA could be detected in the majority (11 of 17, 65%) of non-mantle-cell lymphomas and in a minority of atypical lymphoid hyperplasias (3 of 7, 43%). Cyclin D1 mRNA expression was not observed in floridly reactive lymph nodes (0 of 9) or in unstimulated lymph nodes (0 of 20), suggesting that it is a sensitive adjunct marker for malignant lymphoproliferative processes, but not specific for mantle-cell lymphoma. A semiquantitative RT-PCR assay was developed that compared the ratio of cyclin D1 to the constitutively expressed gene beta2-microglobulin. Using this assay on a limited number of our specimens, cyclin D1 overexpression in mantle-cell lymphoma could be reliably distinguished from its expression in other non-Hodgkin's lymphomas. This assay for cyclin D1 expression, designed for formalin-fixed, paraffin-embedded tissue, was a very sensitive and specific marker for mantle-cell lymphoma.
N S Aguilera; K E Bijwaard; B Duncan; A E Krafft; W S Chu; S L Abbondanzo; J H Lichy; J K Taubenberger
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The American journal of pathology     Volume:  153     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1998-12-29     Completed Date:  1998-12-29     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1969-76     Citation Subset:  AIM; IM    
Department of Hematologic and lymphatic Pathology, Armed Forces Institute of Pathology, Washington, DC 20306-6000, USA.
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MeSH Terms
Aged, 80 and over
Antigens, CD5 / metabolism
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 14
Cyclin D1 / metabolism*
Gene Expression Regulation, Neoplastic*
Immunoglobulin Heavy Chains / genetics
Lymphoma, Non-Hodgkin / metabolism*
Middle Aged
Paraffin Embedding
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tissue Fixation
Translocation, Genetic
beta 2-Microglobulin / metabolism
Reg. No./Substance:
0/Antigens, CD5; 0/Immunoglobulin Heavy Chains; 0/RNA, Messenger; 0/beta 2-Microglobulin; 136601-57-5/Cyclin D1

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