Document Detail

Differential expression and cellular localization of ZNF224 and ZNF255, two isoforms of the Krüppel-like zinc-finger protein family.
MedLine Citation:
PMID:  17900823     Owner:  NLM     Status:  MEDLINE    
We previously reported that ZNF224, a novel Krüppel-associated box-containing zinc-finger protein, represses aldolase A gene transcription by interacting with the KAP-1 co-repressor. Using northern blot and PCR procedures, we now demonstrate that the transcript encoding ZNF255 is a ZNF224 isoform and that the corresponding mRNAs are differentially expressed in human adult and foetal tissues. Moreover, transient transfection of recombinant ZNF224 and ZNF255 proteins and chromatin-immunoprecipitation assays indicate that ZNF224 binds the negative regulatory element of the aldolase A gene (AldA-NRE) and inhibits transcription more efficiently than ZNF255. Finally, ZNF224 was homogeneously distributed in the nucleus, whereas its isoform ZNF255 was identified in subnuclear structures in association with nucleoli, and also in the cytoplasm. The different repression of transcription and the different cellular localization of ZNF224 and ZNF255 suggest these proteins exert different biological role.
Lina Medugno; Francesca Florio; Elena Cesaro; Michela Grosso; Angelo Lupo; Paola Izzo; Paola Costanzo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-30
Journal Detail:
Title:  Gene     Volume:  403     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-05     Completed Date:  2007-11-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  125-31     Citation Subset:  IM    
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli "Federico II", Via S. Pansini 5, 80131 Napoli, Italy.
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MeSH Terms
Blotting, Northern
Cell Line
Cell Nucleolus / metabolism*
Cell Nucleus / metabolism*
Chromatin Immunoprecipitation
Cytoplasm / metabolism*
DNA-Binding Proteins / genetics,  metabolism*
Embryo, Mammalian
Fluorescent Dyes
Fructose-Bisphosphate Aldolase / genetics
Gene Expression Regulation
Hela Cells
Kidney / cytology
Models, Genetic
Polymerase Chain Reaction
Protein Isoforms
RNA, Messenger / metabolism
Recombinant Proteins / metabolism
Regulatory Sequences, Nucleic Acid
Repressor Proteins / genetics,  metabolism*
Zinc Fingers / genetics*
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Fluorescent Dyes; 0/Indoles; 0/Protein Isoforms; 0/RNA, Messenger; 0/Recombinant Proteins; 0/Repressor Proteins; 0/ZNF224 protein, human; 47165-04-8/DAPI; EC Aldolase

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