Document Detail

Differential expression and cellular distribution of centrin isoforms during human ciliated cell differentiation in vitro.
MedLine Citation:
PMID:  10725219     Owner:  NLM     Status:  MEDLINE    
Centrin protein is an ubiquitously expressed cytoskeletal component and is a member of the EF-hand superfamily of calcium-binding proteins. It was first discovered in the flagellar apparatus of unicellular green algae where it is involved in contraction of Ca(2+)-sensitive structures. Centrin protein is associated with centrosome-related structures such as spindle pole body in yeast, and centriole/basal bodies in flagellar and ciliated cells. Three centrin genes have been cloned in human cells. In this work, we have performed a comparative biochemical and functional analysis of centrin isoforms using a primary culture of human nasal epithelial cells which provides an efficient way to obtain a complete ciliated cell differentiation process. RT-PCR experiments show that the expression of the three human centrin genes increases during cell differentiation, and that only centrin 2 and 3 are expressed during cell proliferation. Using polyclonal antibodies raised against recombinant human centrin 2 and 3, we show a specific pattern of protein expression. Ultrastructural immunolocalization suggests that centrin proteins are involved in the early process of centriole assembly, as they are concentrated within the precursor structures of centriole/basal bodies. It also shows a differential localisation of centrin proteins in mature centriole/basal bodies, suggesting different functions for centrins 1/2 and centrin 3. This is also supported by functional analyses showing that centrin 1 and/or centrin 2 are involved in ciliary beating.
J Laoukili; E Perret; S Middendorp; O Houcine; C Guennou; F Marano; M Bornens; F Tournier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  113 ( Pt 8)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-07-31     Completed Date:  2000-07-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1355-64     Citation Subset:  IM    
Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Université Paris 7, France.
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MeSH Terms
Calcium-Binding Proteins / metabolism*
Cell Cycle Proteins
Cell Differentiation
Cells, Cultured
Chromosomal Proteins, Non-Histone / metabolism*
Cilia / metabolism,  ultrastructure
Epithelial Cells / cytology,  metabolism*,  ultrastructure
Microscopy, Electron
Protein Isoforms / metabolism
Reg. No./Substance:
0/CETN2 protein, human; 0/CETN3 protein, human; 0/Calcium-Binding Proteins; 0/Cell Cycle Proteins; 0/Chromosomal Proteins, Non-Histone; 0/Protein Isoforms; 118216-31-2/caltractin

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