| Differential expression and cellular distribution of centrin isoforms during human ciliated cell differentiation in vitro. | |
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MedLine Citation:
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PMID: 10725219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Centrin protein is an ubiquitously expressed cytoskeletal component and is a member of the EF-hand superfamily of calcium-binding proteins. It was first discovered in the flagellar apparatus of unicellular green algae where it is involved in contraction of Ca(2+)-sensitive structures. Centrin protein is associated with centrosome-related structures such as spindle pole body in yeast, and centriole/basal bodies in flagellar and ciliated cells. Three centrin genes have been cloned in human cells. In this work, we have performed a comparative biochemical and functional analysis of centrin isoforms using a primary culture of human nasal epithelial cells which provides an efficient way to obtain a complete ciliated cell differentiation process. RT-PCR experiments show that the expression of the three human centrin genes increases during cell differentiation, and that only centrin 2 and 3 are expressed during cell proliferation. Using polyclonal antibodies raised against recombinant human centrin 2 and 3, we show a specific pattern of protein expression. Ultrastructural immunolocalization suggests that centrin proteins are involved in the early process of centriole assembly, as they are concentrated within the precursor structures of centriole/basal bodies. It also shows a differential localisation of centrin proteins in mature centriole/basal bodies, suggesting different functions for centrins 1/2 and centrin 3. This is also supported by functional analyses showing that centrin 1 and/or centrin 2 are involved in ciliary beating. |
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Authors:
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J Laoukili; E Perret; S Middendorp; O Houcine; C Guennou; F Marano; M Bornens; F Tournier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cell science Volume: 113 ( Pt 8) ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-07-31 Completed Date: 2000-07-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1355-64 Citation Subset: IM |
Affiliation:
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Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Université Paris 7, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calcium-Binding Proteins
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metabolism* Cell Cycle Proteins Cell Differentiation Cells, Cultured Chromosomal Proteins, Non-Histone / metabolism* Cilia / metabolism, ultrastructure Epithelial Cells / cytology, metabolism*, ultrastructure Humans Microscopy, Electron Protein Isoforms / metabolism |
| Chemical | |
Reg. No./Substance:
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0/CETN2 protein, human; 0/CETN3 protein, human; 0/Calcium-Binding Proteins; 0/Cell Cycle Proteins; 0/Chromosomal Proteins, Non-Histone; 0/Protein Isoforms; 118216-31-2/caltractin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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