Document Detail

Differential expression of c-jun and c-fos mRNAs in squamous cell carcinoma of the head and neck: associations with uPA, gelatinase B, and matrilysin mRNAs.
MedLine Citation:
PMID:  11774399     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Head and neck squamous cell carcinomas (HNSCC) are known for their invasive behavior. The invasiveness of these tumors requires proteases, some of which as urokinase-type plasminogen activator (uPA), gelatinase B and matrilysin are regulated through AP-1 dependent transcriptional mechanisms. AP-1 consists of several proteins, including those encoded by the proto-oncogenes c-jun and c-fos. The aim of this study was to: first, evaluate the expression levels of matrix metalloproteases (matrilysin and gelatinase B) and uPA mRNAs; second, examine whether these genes might be associated with c-jun and c-fos expression; third, examine the relationship between the expression of these genes and HNSCC clinico-pathological features. METHODS: We have analyzed 38 HNSCC primary tumors and matched mucosa tissues for uPA, gelatinase B, matrilysin, c-fos, and c-jun by Northern-blot analysis. RESULTS: uPA, gelatinase B, matrilysin, and c-jun mean levels were statistically higher in the tumors than in the normal adjacent mucosa, whereas no difference was found when c-fos mRNA values were compared, c-jun mRNA expression correlated directly with gelatinase B and matrilysin mRNA levels, but no association with uPA mRNA was observed, c-fos mRNA levels were not associated with the tested proteases, but low levels were determined in tumors from older patients who subsequently developed a 2(nd) tumor. No evidence of correlation between expression of uPA, matrilysin, and c-jun in tumors and clinico-pathological features was found. Gelatinase B mRNA high levels were associated to presence of cervical recurrences. CONCLUSION: Expression of c-jun seems to be involved in the regulation of gelatinase B and matrilysin being not related to uPA. Lack of association with c-fos may indicate that other fos family members might play a role in the transcriptional activity of the analyzed proteases in HNSCC tumors.
Mercia Medeiros Pacheco; Luis Paulo Kowalski; Ines Nobuko Nishimoto; Maria Mitzi Brentani
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Head & neck     Volume:  24     ISSN:  1043-3074     ISO Abbreviation:  Head Neck     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-04     Completed Date:  2002-02-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8902541     Medline TA:  Head Neck     Country:  United States    
Other Details:
Languages:  eng     Pagination:  24-32     Citation Subset:  IM    
Copyright Information:
Copyright 2002 John Wiley & Sons, Inc.
Fundação Oncocentro de São Paulo, Brazil.
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MeSH Terms
Aged, 80 and over
Biopsy, Needle
Blotting, Northern
Carcinoma, Squamous Cell / chemistry*,  pathology
Culture Techniques
Gene Expression Regulation, Neoplastic
Head and Neck Neoplasms / chemistry*,  pathology
Matrix Metalloproteinase 7 / genetics
Matrix Metalloproteinase 9 / genetics
Middle Aged
Neoplasm Invasiveness
Proto-Oncogene Proteins c-fos / genetics*
Proto-Oncogene Proteins c-jun / genetics*
RNA, Messenger / analysis*
RNA, Neoplasm / analysis*
Sensitivity and Specificity
Statistics, Nonparametric
Tumor Markers, Biological / analysis*
Urokinase-Type Plasminogen Activator / genetics
Reg. No./Substance:
0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Tumor Markers, Biological; EC Plasminogen Activator; EC Metalloproteinase 7; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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