Document Detail

Differential expression between synaptic vesicle proteins and presynaptic plasma membrane proteins in the anterior horn of amyotrophic lateral sclerosis.
MedLine Citation:
PMID:  11810185     Owner:  NLM     Status:  MEDLINE    
This study concerns the immunohistochemical investigation of synaptic proteins in the anterior horn of amyotrophic lateral sclerosis (ALS). Antibodies against synapsin 1 and synaptophysin (i.e. synaptic vesicle proteins), and those against syntaxin and the synaptosomal-associated, 25 kDa protein, SNAP25 (i.e. presynaptic plasma membrane proteins) were used for immunostaining, respectively. Lumbar spinal cords from five ALS and eight control patients were examined. In the controls, all four synaptic proteins exhibited fine granular immunoreactivities, distributed throughout the spinal gray matter almost uniformly. In contrast, in all five ALS patients, two of the synaptic vesicle proteins examined decreased in the anterior horn neuropil diffusely, while in the same lumbar segments of these cases the immunoreactivities of the two presynaptic plasma membrane proteins showed no apparent decrease, or were only mildly diminished in the same gray matter area. These results indicate that, during the presynaptic terminal degeneration in the anterior horn of ALS, synaptic vesicle involvement may precede that of the presynaptic plasma membrane.
Akito Ikemoto; Shinichi Nakamura; Ichiro Akiguchi; Asao Hirano
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Publication Detail:
Type:  Journal Article     Date:  2001-11-10
Journal Detail:
Title:  Acta neuropathologica     Volume:  103     ISSN:  0001-6322     ISO Abbreviation:  Acta Neuropathol.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-01-25     Completed Date:  2002-05-23     Revised Date:  2007-11-09    
Medline Journal Info:
Nlm Unique ID:  0412041     Medline TA:  Acta Neuropathol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  179-87     Citation Subset:  IM    
Laboratory of Anatomic Pathology, Kyoto University Hospital, 54 Shogoin-Kawaharacho, Sakyoku, Kyoto 606-8507, Japan.
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MeSH Terms
Aged, 80 and over
Amyotrophic Lateral Sclerosis / immunology*,  pathology,  physiopathology
Anterior Horn Cells / immunology*,  pathology,  physiopathology
Cell Membrane / immunology*
Membrane Proteins / immunology*
Middle Aged
Nerve Tissue Proteins / immunology*
Presynaptic Terminals / immunology*,  pathology,  physiology
Reg. No./Substance:
0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/SVP-38 protein, human

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