| Differential expression and activity of p34cdc2 in cultured aortic adventitial fibroblasts derived from spontaneously hypertensive and Wistar-Kyoto rats. | |
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MedLine Citation:
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PMID: 8390519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The present investigation was undertaken to determine whether p34cdc2, a cell-cycle regulatory kinase, is involved in the manifestation of the altered proliferation evident in fibroblasts isolated from spontaneously hypertensive rats (SHR). DESIGN: Experiments were performed on quiescent aortic adventitial fibroblasts stimulated to re-enter the cell cycle in order to examine the timing of cell cycle-related events. METHODS: The cell-cycle phase was determined by flow cytometry and was related to the cellular content and kinase activity of p34cdc2. RESULTS: SHR fibroblasts displayed a heightened basal level of p34cdc2 at quiescence relative to Wistar-Kyoto (WKY) rat cells. Both SHR and WKY fibroblasts showed a cell cycle-dependent increase in p34cdc2 content, beginning in S phase. However, the SHR adventitial fibroblasts exited G0-G1 several hours earlier than the WKY fibroblasts as indicated by the time of initiation of DNA synthesis and increase in activity of p34cdc2. CONCLUSIONS: SHR aortic adventitial fibroblasts appear to have a heightened proliferative capacity relative to WKY fibroblasts, which is evident in a quicker exit from G0 and faster transition to DNA synthesis, followed by the earlier activation of p34cdc2. |
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Authors:
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S L Venance; M H Watson; D A Wigle; A S Mak; S C Pang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of hypertension Volume: 11 ISSN: 0263-6352 ISO Abbreviation: J. Hypertens. Publication Date: 1993 May |
Date Detail:
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Created Date: 1993-07-22 Completed Date: 1993-07-22 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8306882 Medline TA: J Hypertens Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 483-9 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Queen's University, Kingston, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / enzymology* CDC2 Protein Kinase / analysis*, metabolism Cell Cycle Cells, Cultured DNA / biosynthesis Fibroblasts / enzymology Hypertension / enzymology* Male Rats Rats, Inbred SHR Rats, Inbred WKY |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA; EC 2.7.11.22/CDC2 Protein Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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