| Differential expression of WT1 gene product in non-Hodgkin lymphomas. | |
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MedLine Citation:
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PMID: 15894924 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The tumor suppressor gene wt1 (Wilms tumor 1) encodes a zinc finger transcription factor reported to be expressed in many tumors, including mesotheliomas, carcinomas, and acute leukemias. However, WT1 expression in non-Hodgkin lymphomas (NHLs) has not been studied. The authors assessed for WT1 expression in six lymphoma/leukemia cell lines using Western blot methods after subcellular fractionation. We also assessed for WT1 expression in 167 NHLs using immunohistochemical methods. The B-cell NHLs analyzed were 18 diffuse large B-cell lymphomas, 13 marginal zone B-cell lymphomas, 9 small lymphocytic lymphomas, (DLBCLs), 8 follicular lymphomas, 6 mantle cell lymphomas, 5 Burkitt lymphomas, 3 lymphoplasmacytic lymphomas, and 2 B-cell lymphoblastic lymphomas. The T-cell NHLs analyzed were 43 anaplastic large cell lymphomas (ALCLs), 26 peripheral T-cell lymphomas unspecified, 13 angioimmunoblastic T-cell lymphomas, 6 cutaneous ALCLs, 6 cases of mycosis fungoides, 5 extranodal NK/T-cell lymphomas of nasal type, and 4 T-cell lymphoblastic lymphomas. WT1 levels were higher in cytoplasmic extracts than in nuclear extracts of the Karpas 299 and SU-DHL-1 lymphoma cell lines but were higher in nuclear extracts than in the cytoplasmic extracts of the Jurkat, HH, U-937, and K562 leukemia cell lines. In NHLs, WT1 was positive in 4 of 5 (80%) Burkitt lymphomas, 9 of 12 (75%) ALK-positive ALCLs, 3 of 6 (50%) lymphoblastic lymphomas (2 of 4 T-cell, 1 of 2 B-cell), 14 of 31 (45%) ALK-negative ALCLs, 6 of 18 (33%) DLBCLs, and 1 of 6 (17%) cutaneous ALCLs. WT1 was negative in all other NHLs tested. WT1 immunoreactivity was primarily cytoplasmic in all positive NHLs except T-cell lymphoblastic lymphoma. In conclusion, WT1 protein is frequently detected in the cytoplasm of a subset of high-grade NHLs. |
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Authors:
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Elias Drakos; George Z Rassidakis; Panagiota Tsioli; Raymond Lai; Dan Jones; L Jeffrey Medeiros |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry Volume: 13 ISSN: 1541-2016 ISO Abbreviation: Appl. Immunohistochem. Mol. Morphol. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-05-16 Completed Date: 2005-08-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888796 Medline TA: Appl Immunohistochem Mol Morphol Country: United States |
Other Details:
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Languages: eng Pagination: 132-7 Citation Subset: IM |
Affiliation:
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Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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B-Lymphocytes
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metabolism,
pathology Blotting, Western Cell Line, Tumor Cytoplasm / metabolism* Humans Immunohistochemistry Lymphoma, Non-Hodgkin / genetics*, metabolism T-Lymphocytes / metabolism, pathology WT1 Proteins / biosynthesis, genetics* |
| Chemical | |
Reg. No./Substance:
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0/WT1 Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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