Document Detail


Differential expression of a WD protein during squamous differentiation of tracheal epithelial cells.
MedLine Citation:
PMID:  12112030     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lining of the trachea consists of a pseudostratified, mucociliary epithelium that under a variety of conditions, such as vitamin A deficiency, toxic and mechanical injury, becomes a stratified squamous epithelium. Several in vitro cell culture models have been established to study the process of differentiation of airway epithelium. Such studies have indicated that mucosecretory differentiation of tracheal epithelial cells can be modulated by substratum. This study was undertaken to understand molecular mechanisms of squamous differentiation in tracheal epithelia. Primary cultured tracheal cells grown on uncoated filters were differentiated to single layer of squamous cells, whereas cells were grown as stratified columnar cells on collagen-I coated filters. The responses to secretagogues were altered according to culture conditions. DD-PCR revealed that FAK and a WD protein expression was increased in squamous tracheal epithelia. Expression of a WD protein was changed by the treatment of retinoic acid in various epithelial cells. These results indicated that squamous differentiation of tracheal cells changes the expression of a variety of genes, and that the experimental model for this study can be employed to study molecular mechanisms of squamous differentiation in airway epithelial cells.
Authors:
Y S Kim; S M Hwang; S J Lee; J S Jung
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  86     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2002  
Date Detail:
Created Date:  2002-07-11     Completed Date:  2002-12-17     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  194-201     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Wiley-Liss, Inc.
Affiliation:
Department of Microbiology, College of Natural Sciences, Pusan National University, Pusan 602-739, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Culture Techniques
Cell Differentiation*
Cell Size
Cells, Cultured
Collagen Type I / metabolism
Culture Media / chemistry
DNA, Complementary / genetics
Epithelial Cells / cytology*,  drug effects,  metabolism*
Focal Adhesion Kinase 1
Focal Adhesion Protein-Tyrosine Kinases
Gene Expression Profiling*
Gene Expression Regulation, Developmental* / drug effects
Gene Library
Molecular Sequence Data
Polymerase Chain Reaction
Protein-Tyrosine Kinases / metabolism
Proteins / genetics*
Rats
Rats, Sprague-Dawley
Trachea / cytology*,  drug effects,  metabolism*
Tretinoin / pharmacology
Chemical
Reg. No./Substance:
0/Collagen Type I; 0/Culture Media; 0/DNA, Complementary; 0/Proteins; 302-79-4/Tretinoin; EC 2.7.10.1/Focal Adhesion Kinase 1; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.2/Focal Adhesion Protein-Tyrosine Kinases; EC 2.7.10.2/Ptk2 protein, rat
Comments/Corrections
Erratum In:
J Cell Biochem 2002;87(3):360

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