| Differential expression of T cell differentiation antigens and major histocompatibility antigens on activated T cells during the cell cycle. | |
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MedLine Citation:
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PMID: 3082649 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this report we have analyzed cell cycle-related fluctuations of both quantity and density of the T cell differentiation antigens, CD3 (T3), CD4 (T4) and CD8 (T8), as well as the major histocompatibility complex (MHC) antigens on the cell surface of activated T cells. Phytohemagglutinin-activated T cells cultured for 3 days with or without conditioned medium or for 10 days with conditioned medium and mixed lymphocyte culture-derived T cell clones were used for the analysis. Correlated measurements of the surface antigen quantity (immunofluorescence), DNA content (dye Hoechst 33342), and cell size (light scatter), not influenced by synchrony induction methods and cell fixation, were performed by dual-beam flow cytometry. Our results demonstrate that the T cell differentiation antigens, CD3, CD4 and CD8, and class I MHC antigens are increased in density in the G1 phase for all activated T cells tested. In contrast, class II MHC antigens are increased in density in the G2 phase of activated T cells maintained with conditioned medium. Since it is known that the T cell differentiation antigens and class I MHC antigens on activated T cells are necessary for proliferation of T cells, our study suggests that this effect is more significant in the G1 phase. The cell cycle changes in expression of class I and class II MHC antigens, but not of the T cell differentiation antigens, appear to be mediated by soluble factors, probably including interferon-gamma, which could produce a differential increase of class I and class II MHC antigens on G2 phase cells. |
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Authors:
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Y Matsui; H M Shapiro; M J Sheehy; L Christenson; D E Staunton; E E Eynon; E J Yunis |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: European journal of immunology Volume: 16 ISSN: 0014-2980 ISO Abbreviation: Eur. J. Immunol. Publication Date: 1986 Mar |
Date Detail:
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Created Date: 1986-05-12 Completed Date: 1986-05-12 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 1273201 Medline TA: Eur J Immunol Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 248-51 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Differentiation, T-Lymphocyte Antigens, Surface / analysis* Cell Cycle* DNA / analysis Gene Expression Regulation HLA Antigens / analysis* Histocompatibility Antigens Class II / analysis* Humans Lymphocyte Activation* / drug effects Lymphokines / physiology Phytohemagglutinins / pharmacology T-Lymphocytes / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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AI 17120/AI/NIAID NIH HHS; CA 06516/CA/NCI NIH HHS; CA 20531/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Differentiation, T-Lymphocyte; 0/Antigens, Surface; 0/HLA Antigens; 0/Histocompatibility Antigens Class II; 0/Lymphokines; 0/Phytohemagglutinins; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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