Document Detail


Differential expression of MUC1 and carbohydrate antigens in primary and secondary head and neck squamous cell carcinoma.
MedLine Citation:
PMID:  18302268     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: In head and neck squamous cell carcinoma (HNSCC), tumor markers may be helpful to evaluate prognosis accurately as well as to improve therapy selection. Detection of human MUC1 has been widely employed for the evaluation of carcinoma patients. This article aims to study MUC1, Tn, sTn, and Lewis antigenic expression in primary HNSCC, lymph node metastasis, and local recurrences. METHODS: We used immunohistochemistry, tissue homogenization and differential centrifugation, isopycnic density gradient centrifugation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western blot. RESULTS: In primary tumors, MUC1 was detected in 80.0% of the samples; sLewis x in 23.2%, Lewis x in 45.6%, and Lewis y in 40.8%. Tn and sTn were found in 4.0% and 6.4% of samples, respectively. In metastatic lymph nodes, MUC1 showed a similar positive reaction as in primary tumors. Lewis y was detected in 20% lymph nodes whereas Lewis x, sLewis x, Tn, and sTn did not show differences. Some recurrences expressed MUC1 and only a few Lewis antigens, whereas Tn and sTn were not detected. CONCLUSION: In primary HNSCC and metastatic nodes, a high expression of MUC1 and Lewis antigens was detected that diminished in local recurrences. We also found that differentiated tumors mainly expressed a linear pattern of MUC1CT and Lewis x.
Authors:
María V Croce; Martín E Rabassa; Adrián Pereyra; Amada Segal-Eiras
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Head & neck     Volume:  30     ISSN:  1043-3074     ISO Abbreviation:  Head Neck     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-16     Completed Date:  2008-11-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8902541     Medline TA:  Head Neck     Country:  United States    
Other Details:
Languages:  eng     Pagination:  647-57     Citation Subset:  IM    
Affiliation:
Faculty of Medical Sciences, Centre of Basic and Applied Immunological Research (CINIBA),UNLP, Calle 60 y 120, 1900 La Plata, Argentina. crocevir@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Antigens, Tumor-Associated, Carbohydrate / metabolism*
Blotting, Western
Carcinoma, Squamous Cell / immunology*,  metabolism*
Epitopes / metabolism
Female
Head and Neck Neoplasms / immunology*,  metabolism*
Humans
Immunohistochemistry
Lymph Nodes / immunology
Lymphatic Metastasis
Male
Middle Aged
Mucin-1 / metabolism*
Neoplasm Recurrence, Local / immunology,  metabolism
Tumor Markers, Biological
Chemical
Reg. No./Substance:
0/Antigens, Tumor-Associated, Carbohydrate; 0/Epitopes; 0/MUC1 protein, human; 0/Mucin-1; 0/Tumor Markers, Biological

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