Document Detail


Differential expression of LFA-3, Fas and MHC Class I on Ad5- and Ad12-transformed human cells and their susceptibility to lymphokine-activated killer (LAK) cells.
MedLine Citation:
PMID:  15963548     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adenovirus (Ad) E1A is a potent oncogene and has been shown to deregulate the expression of a large number of cellular genes leading to cellular transformation. Here we have analysed the expression of several immunomodulatory molecules on the surface of a set of human cell lines transformed with either Ad12 or Ad5. Human cells transformed with Ad12 demonstrated reduced expression of cell surface LFA-3, Fas and MHC class I when compared to Ad5-transformed cells. Furthermore, Ad12-transformed human cell lines demonstrated greater susceptibility to lysis by lymphokine-activated killer (LAK) cells, compared to Ad5-transformed human cell lines. In contrast, previous studies with rodent cells showed that both Ad5- and Ad12-transformed rat cells were susceptible to LAK cells. Thus, transformation of human cells with Ad5 or Ad12 results in differences in the expression of immunomodulatory molecules on the cell surface and differential recognition of these virus-transformed cells by immune effector cells.
Authors:
Graham Bottley; Graham P Cook; Josephine L Meade; John R Holt; Rob C Hoeben; G Eric Blair
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virology     Volume:  338     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-25     Completed Date:  2005-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  297-308     Citation Subset:  IM    
Affiliation:
Molecular Cell Biology Research Group and School of Biochemistry and Microbiology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics*
Adenovirus E1A Proteins / genetics
Adenovirus E1B Proteins / genetics
Antigens, CD58 / genetics*
Antigens, CD95 / genetics*
Base Sequence
Cell Line, Transformed
Cell Line, Tumor
DNA Primers
Gene Expression Regulation / immunology*
Histocompatibility Antigens Class I / genetics
Humans
Killer Cells, Lymphokine-Activated / immunology*,  virology*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Adenovirus E1A Proteins; 0/Adenovirus E1B Proteins; 0/Antigens, CD58; 0/Antigens, CD95; 0/DNA Primers; 0/Histocompatibility Antigens Class I

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