Document Detail


Differential expression of H19 and vitamin D3 upregulated protein 1 as a mechanism of the modulatory effects of high virgin olive oil and high corn oil diets on experimental mammary tumours.
MedLine Citation:
PMID:  19337063     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dietary lipids have a role in the aetiology of breast cancer. We have reported earlier that a high corn oil diet downregulates H19 and vitamin D3 upregulated protein 1 (VDUP1) messenger RNA (mRNA) expression in rat dimethylbenz (alpha) anthracene-induced mammary adenocarcinomas in comparison with the control low-fat diet, this effect being associated with a higher degree of tumour malignancy. This result was compatible with the stimulating effect of this diet. In this study we have investigated the influence of a high extra virgin olive diet on H19 and VDUP1 mRNA and/or protein expression. We have shown earlier that this high-fat diet confers to the tumours a more benign phenotype in accordance with its potential protective effect on mammary cancer. We have also analysed the effects on the mRNA and protein expression of insulin-like growth factor-2 , in close relation with H19, and the expression and activity of the thioredoxin protein, negatively regulated by VDUP1. mRNA and protein expression were analysed by chemiluminescent northern blot and western blot, respectively. Thioredoxin activity was determined by the insulin-reducing assay. The results showed that the high olive oil diet does not change the tumour expression of H19 and VDUP1. Moreover, tumours from the animals fed this diet displayed higher levels of the insulin-like growth factor-2 mRNAs, which are related to a higher rate of degradation or a lower traducibility. Finally, tumour expression and activity levels of thioredoxin-1 protein did not change irrespective of the diet. These results suggest that the differential effects of high olive oil and high corn oil diets on mammary cancer are exerted by means of a different, specific influence on gene expression.
Authors:
Montserrat Solanas; Raquel Moral; Gemma Garcia; Laura Grau; Elena Vela; Raquel Escrich; Irmgard Costa; Eduard Escrich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)     Volume:  18     ISSN:  1473-5709     ISO Abbreviation:  Eur. J. Cancer Prev.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-01     Completed Date:  2009-07-27     Revised Date:  2011-10-07    
Medline Journal Info:
Nlm Unique ID:  9300837     Medline TA:  Eur J Cancer Prev     Country:  England    
Other Details:
Languages:  eng     Pagination:  153-61     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Physiology and Immunology, Medical Physiology Unit, Medicine School, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / diet therapy,  genetics*,  pathology
Animals
Carrier Proteins / genetics*
Corn Oil / pharmacology*
Dietary Fats, Unsaturated / pharmacology,  therapeutic use
Female
Gene Expression Regulation, Neoplastic / drug effects*
Insulin-Like Growth Factor II / genetics
Mammary Neoplasms, Experimental / diet therapy,  genetics*,  pathology
Plant Oils / pharmacology*
RNA, Untranslated / genetics*
Rats
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Dietary Fats, Unsaturated; 0/H19 long non-coding RNA; 0/Plant Oils; 0/RNA, Untranslated; 0/TXNIP protein, rat; 67763-97-7/Insulin-Like Growth Factor II; 8001-25-0/olive oil; 8001-30-7/Corn Oil

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