Document Detail

Differential expression of E-cadherin at the surface of rat beta-cells as a marker of functional heterogeneity.
MedLine Citation:
PMID:  17592017     Owner:  NLM     Status:  MEDLINE    
The aim of this study was to assess whether the expression of E-cadherin at the surface of rat beta-cells is regulated by insulin secretagogues and correlates with insulin secretion. When cultured under standard conditions, virtually all beta-cells expressed E-cadherin observed by immunofluorescence, but heterogeneous staining was observed. Using fluorescence-activated cell sorting (FACS), two beta-cell sub-populations were sorted: one that was poorly labeled ('ECad-low') and another that was highly labeled ('ECad-high'). After 1-h stimulation with 16.7 mM glucose, insulin secretion (reverse hemolytic plaque assay) from individual ECad-high beta-cells was higher than that from ECad-low beta-cells. Ca2+-dependent beta-cell aggregation was increased at 16.7 mM glucose when compared with 2.8 mM glucose. E-cadherin at the surface of beta-cells was increased after 18 h at 11.1 and 22.2 mM glucose when compared with 2.8 mM glucose, with the greatest increase at 22.2 mM glucose + 0.5 mM isobutylmethylxanthine (IBMX). While no labeling was detected on freshly trypsinized cells, the proportion of stained cells increased in a time-dependent manner during culture for 1, 3, and 24 h. This recovery was faster when cells were incubated at 16.7 vs 2.8 mM glucose. Cycloheximide inhibited expression of E-cadherin at 2.8 mM glucose, but not at 16.7 mM, while depolymerization of actin by either cytochalasin B or latrunculin B increased surface E-cadherin at low glucose. In conclusion, these results show that expression of E-cadherin at the surface of islet beta-cells is controlled by secretagogues including glucose, correlates with insulin secretion, and can serve as a surface marker of beta-cell function.
Domenico Bosco; Dominique G Rouiller; Philippe A Halban
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  194     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-26     Completed Date:  2007-09-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  21-9     Citation Subset:  IM    
Surgical Research Unit, Department of Surgery, Cell Isolation and Transplantation Center, CMU, Geneva University Hospitals, 1, rue Michel-Servet, 1211 Geneva-4, Switzerland.
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MeSH Terms
1-Methyl-3-isobutylxanthine / pharmacology
Actins / metabolism
Bicyclo Compounds, Heterocyclic / pharmacology
Biological Markers / analysis
Cadherins / analysis,  metabolism*
Cells, Cultured
Cycloheximide / pharmacology
Cytochalasin B / pharmacology
Cytotoxins / pharmacology
Flow Cytometry
Fluorescent Antibody Technique
Glucose / metabolism,  pharmacology
Insulin / secretion*
Islets of Langerhans / chemistry,  metabolism*
Marine Toxins / pharmacology
Phosphodiesterase Inhibitors / pharmacology
Protein Synthesis Inhibitors / pharmacology
Rats, Sprague-Dawley
Thiazolidines / pharmacology
Time Factors
Reg. No./Substance:
0/Actins; 0/Bicyclo Compounds, Heterocyclic; 0/Biological Markers; 0/Cadherins; 0/Cytotoxins; 0/Marine Toxins; 0/Phosphodiesterase Inhibitors; 0/Protein Synthesis Inhibitors; 0/Thiazolidines; 11061-68-0/Insulin; 14930-96-2/Cytochalasin B; 28822-58-4/1-Methyl-3-isobutylxanthine; 50-99-7/Glucose; 66-81-9/Cycloheximide; 76343-94-7/latrunculin B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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