| Differential effects of three preparations of human serum on expansion of various types of human cells. | |
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MedLine Citation:
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PMID: 18626359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: To avoid potential risks of animal-derived products such as viral transmission and immunologic reactions, usefulness of human-derived products in manipulation of cells for cell-based therapies has been investigated but has not yet been completely clarified. METHODS: Three types of human sera-serum from whole blood, serum from platelet-rich plasma, and serum from platelet-poor plasma-were prepared from blood samples obtained from the same four volunteers. The authors investigated the biochemical profiles of the three serum preparations as well as their potential as culture additives using three types of human cells: dermal fibroblasts, adipose-derived stem/stromal cells, and umbilical vein endothelial cells. RESULTS: Platelet counts differed among serum from whole blood (100 percent), platelet-rich plasma (75.1 percent), and platelet-poor plasma (12.6 percent), resulting in differential concentrations of platelet-derived growth factor and epidermal growth factor, although other biochemical values such as total protein and albumin were similar. Serum from whole blood and platelet-rich plasma highly enhanced proliferation of dermal fibroblasts compared with the effects of serum from platelet-poor plasma, but no differences in proliferative efficacy were observed in cultures of adipose-derived stem/stromal cells and vascular endothelial cells. CONCLUSIONS: Serum from platelet-rich plasma, which is less invasive to prepare than serum from whole blood, was superior to serum from platelet-poor plasma as a substitute for animal-derived serum in culture expansion of dermal fibroblasts. Although autologous or human-derived serum preparations may be of great use in cell-based therapies, this usefulness strongly depends on the target cell species and the purpose of the cell culture. |
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Authors:
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Masakazu Kurita; Emiko Aiba-Kojima; Tomokuni Shigeura; Daisuke Matsumoto; Hirotaka Suga; Keita Inoue; Hitomi Eto; Harunosuke Kato; Noriyuki Aoi; Kotaro Yoshimura |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Plastic and reconstructive surgery Volume: 122 ISSN: 1529-4242 ISO Abbreviation: Plast. Reconstr. Surg. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2010-07-27 Completed Date: 2010-08-16 Revised Date: 2011-02-16 |
Medline Journal Info:
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Nlm Unique ID: 1306050 Medline TA: Plast Reconstr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 438-48 Citation Subset: AIM; IM |
Affiliation:
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Department of Plastic Surgery, University of Tokyo School of Medicine, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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cytology* Adult Cell Division / physiology* Culture Media, Conditioned* Endothelial Cells / cytology* Epidermal Growth Factor / blood Female Fibroblasts / cytology* Humans Male Platelet Count* Platelet-Derived Growth Factor / metabolism Platelet-Rich Plasma* Serum* Stem Cells / cytology* Stromal Cells / cytology* Tissue Engineering / methods* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Platelet-Derived Growth Factor; 62229-50-9/Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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