| Differential effects of nicotine on retinal and vascular cells in vitro. | |
| | |
MedLine Citation:
|
PMID: 19428945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The purpose of the current study is to understand the effects of nicotine in human retinal pigment epithelial (ARPE-19), human microvascular endothelial cells (HMVEC) and rat neurosensory retinal (R28) cells. ARPE-19, HMVEC and R28 cell cultures were treated with 10(-2) and 10(-4)M nicotine for 24h. R28 cells were also pre-treated for 4h with ALLN and ALLM (calpain inhibitors) or epicatechin, an antioxidant flavonoid compound. Trypan blue dye exclusion assay, caspase-3/7, LDH activity and DNA laddering assays were performed. With 10(-2)M nicotine treatment, R28 cell cultures showed decreased cell viability that was partially reversed by pre-treatment with the antioxidant epicatechin but not with calpain inhibitors. The DNA ladder assay showed a 200bp banding pattern consistent with apoptosis, however, caspase-3/7 activity was not increased. After treatment with 10(-2)M nicotine, HMVEC cultures showed decreased cell viability and increased LDH activity but no DNA banding patterns or caspase-3/7 activity. ARPE-19 cells showed no change in cell viability or caspase-3/7 activity at any of the nicotine concentrations. We conclude of dissimilar responses to nicotine treatment in three different cell lines. Nicotine was toxic to HMVEC and R28 cell cultures but the ARPE-19 cells were unaffected. In R28 cells, the nicotine effects were through an oxidant pathway that is non-caspase, non-calpain mediated while the HMVEC toxicity was via necrosis. Understanding the mechanisms of cell death may have potential therapeutic implications in the treatment of cigarette smoking related retinal diseases such as age-related macular degeneration (AMD). |
| | |
Authors:
|
A Jayaprakash Patil; Ana L Gramajo; Ashish Sharma; Gail M Seigel; Baruch D Kuppermann; M Cristina Kenney |
Related Documents
:
|
18384095 - Erythrodiol, a natural triterpenoid from olives, has antiproliferative and apoptotic ac... 19802655 - Polyacetylene diols with antiproliferative and driving th1 polarization effects from th... 17105485 - A chemical genetic screen for cell cycle inhibitors in zebrafish embryos. 9661285 - Novel approaches to prodrugs of anticancer diaminodichloroplatinum(ii) complexes activa... 9800195 - Caffeine effect on the mitotic delay induced by g2 treatment with uvc or mitomycin c. 15297025 - Analysis of changes in energy and redox states in hepg2 hepatoma and c6 glioma cells up... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-20 |
Journal Detail:
|
Title: Toxicology Volume: 259 ISSN: 0300-483X ISO Abbreviation: Toxicology Publication Date: 2009 May |
Date Detail:
|
Created Date: 2009-05-11 Completed Date: 2009-06-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0361055 Medline TA: Toxicology Country: Ireland |
Other Details:
|
Languages: eng Pagination: 69-76 Citation Subset: IM |
Affiliation:
|
Department of Ophthalmology, Gavin S. Herbert Eye Institute, University of California Irvine, Medical Center, 101 The City Drive, Orange, CA 92868, United States. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antioxidants / pharmacology Caspase 3 / drug effects, metabolism Caspase 7 / drug effects, metabolism Catechin / pharmacology Cell Line Cell Survival / drug effects Cells, Cultured DNA Fragmentation / drug effects Dose-Response Relationship, Drug Endothelial Cells / drug effects*, metabolism Humans L-Lactate Dehydrogenase / drug effects, metabolism Necrosis / chemically induced Nicotine / administration & dosage, toxicity* Nicotinic Agonists / administration & dosage, toxicity* Rats Retina / cytology, drug effects*, metabolism Retinal Pigment Epithelium / drug effects*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/Nicotinic Agonists; 154-23-4/Catechin; 54-11-5/Nicotine; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 7 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Studies on distribution and metabolism of para-methoxymethamphetamine (PMMA) in rats after subcutane...
Next Document: Inhibition of erythrocyte phosphoribosyltransferases (APRT and HPRT) by Pb2+: a potential mechanism ...